Dissipation of Sulfonamides in Soil Emphasizing Taxonomy and Function of Microbiomes by Metagenomic Analysis
Dissipation of Sulfonamides in Soil Emphasizing Taxonomy and Function of Microbiomes by Metagenomic Analysis
复制标题
磺胺类药物在土壤中的消散，通过宏基因组分析强调微生物组的分类和功能
DOI：
10.1021/acs.jafc.0c04496
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发表时间：
2020-11-25
影响因子：
6.1
通讯作者：
Zou, Xiaoming
中科院分区：
文献类型：
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作者：
Li, Mi;Rong, Lingling;Zou, Xiaoming
磺胺类化合物 (SA) 在土壤中广泛存在，其消散行为对其归宿、风险评估和污染控制非常重要。在这项工作中，我们研究了有氧条件下土壤中不同SA的消散行为，重点揭示了总体消散（未经灭菌和黑暗）与个体非生物（吸附、水解）/生物（灭菌和黑暗）因素之间的关系以及微生物组的分类/功能。结果表明，土壤中所有SA的消散均遵循准一级动力学模型，去除50%时的消散时间（DT50）为2.16-15.27天。基于实验上的非生物/生物过程和理论上的部分最小二乘模型，建立了总体耗散与个体非生物/生物因素之间的关系，其中微生物降解是主要贡献者。宏基因组分析表明，慢生根瘤菌/鞘氨醇单胞菌/甲基阿魏等分类属和 CAZy 家族 GT51/GH23/GT2、eggNOG S 类、KEGG 途径 ko02024/ko02010 和 KEGG 直系同源物 K01999/K03088 等功能可能参与土壤中 SA 微生物的降解。斯皮尔曼相关表明丰富的属具有多功能性。这项研究提供了一些关于 SA 消散的新见解，并且可以在未来应用于其他抗生素/土壤。
Sulfonamides (SAs) are widespread in soils, and their dissipation behavior is important for their fate, risk assessment, and pollution control. In this work, we investigated the dissipation behavior of different SAs in a soil under aerobic condition, focusing on revealing the relationship between overall dissipation (without sterilization and in dark) and individual abiotic (sorption, hydrolysis)/biotic (with sterilization and in dark) factors and taxonomy/function of microbiomes. The results showed that dissipation of all SAs in the soil followed the pseudo-first-order kinetic model with dissipation time at 50% removal (DT50) of 2.16-15.27 days. Based on, experimentally, abiotic/biotic processes and, theoretically, partial least-squares modeling, a relationship between overall dissipation and individual abiotic/biotic factors was developed with microbial degradation as the dominant contributor. Metagenomic analysis showed that taxonomic genera like Bradyrhizobium/Sphingomonas/Methyloferula and functions like CAZy family GT51/GH23/GT2, eggNOG category S, KEGG pathway ko02024/ko02010, and KEGG ortholog K01999/K03088 are putatively involved in SA microbial degradation in soil. Spearman correlation suggests abundant genera being multifunctional. This study provides some new insights into SA dissipation and can be applied to other antibiotics/soils in the future.