Tracking glycosylation in live cells using FTIR spectroscopy.
Tracking glycosylation in live cells using FTIR spectroscopy.
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DOI：
10.1016/j.talanta.2020.120737
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发表时间：
2020-05
期刊：
影响因子：
6.1
通讯作者：
J. Phelan;Ali Altharawi;K. Chan
中科院分区：
文献类型：
--
作者：
J. Phelan;Ali Altharawi;K. Chan
这是首次使用商用四乙酰化n -叠氮多乙酰- d -甘露糖胺（Ac4ManNAz）标签，通过FTIR研究活细胞中的糖蛋白周转。FTIR分析显示能够实时监测活细胞中聚糖的代谢。该方法简单，定量，需要许多实验室可用的设备。它可以用于广泛的应用，如糖基化和细胞信号的研究。
This is the first demonstration of the study of glycan protein turnover in living cells by FTIR with commercially available tetraacetylated N-Azidoacetyl-D-Mannosamine (Ac4ManNAz) label. The FTIR analysis has shown to be able to monitor the metabolism of glycans in living cells in real time. The method is simple, quantitative and requires equipment that are available in many laboratories. It can be used in a wide range of applications such as the study of glycosylation and cell-signalling.