Restoration of lactation in bromocriptine-treated rats by prolactin replacement: comparison of constant versus pulsatile infusion and intrahepatic versus intrajugular routes of delivery.
Restoration of lactation in bromocriptine-treated rats by prolactin replacement: comparison of constant versus pulsatile infusion and intrahepatic versus intrajugular routes of delivery.
复制标题
通过催乳素替代来恢复溴隐亭治疗的大鼠的泌乳：恒定输注与脉冲输注以及肝内与颈内给药途径的比较。
DOI：
10.1007/bf03345825
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发表时间：
1993
影响因子：
5.4
通讯作者：
Nicoll,CS
中科院分区：
文献类型：
--
作者：
Hebert,NJ;Kim,JH;Lin,RJ;Nicoll,CS
比较了脉冲输注与不间断输注绵羊泌乳素（PRL）对泌乳素抑制大鼠恢复泌乳的效果，并探讨了肝脏参与泌乳素恢复作用的可能性。在产后第7天至12天皮下注射溴隐亭（BC）以抑制内源性PRL分泌。采用微型渗透泵将oPRL注入颈静脉或肝门静脉。后一种途径会使肝脏暴露于比静脉注射更高浓度的PRL。总体而言，无论分娩部位如何，持续输注不同剂量的oPRL在恢复泌乳（即窝重增加）方面比给予脉冲更有效。以100 μg/大鼠/天的脉冲持续时间为1小时输注PRL在4次或8次/天的频率下无效，而以2小时的脉冲持续时间在这两个频率下均有效。输注该剂量的oPRL无论是4次还是8次，每次持续2小时，都同样有效。无论给药方案如何，oPRL肝内输注并不比颈静脉输注更有效。这些结果表明，脉冲持续时间比脉冲频率更能决定泌乳大鼠PRL的泌乳效果。没有证据表明肝脏参与了PRL的半乳糖生成作用。
The effectiveness of pulsed vs constant infusion of ovine(o) prolactin (PRL), given by different schedules, at restoring lactation in PRL-suppressed rats was compared, and the possibility that the liver participates in the restorative effects of the infused hormone was investigated. Lactating dams were given subcutaneous injections of bromocriptine (BC) between days 7 and 12 postpartum to suppress endogenous PRL secretion. Osmotic minipumps were used to infuse the oPRL into either the jugular vein or the hepatic portal vein. The latter route would expose the liver to higher concentrations of PRL than would intrajugular infusion. Constant infusion of oPRL in different doses was, overall, more effective at restoring lactation (i.e. litter weight gain) than was giving pulses, regardless of the site of delivery. Infusion of the PRL at 100 μg/rat/day in pulses of 1h duration was ineffective at frequencies of either 4 or 8/day, whereas pulses of 2h duration were effective at both of these frequencies. Infusing that dose of oPRL was equally effective whether it was given in 4 or 8 pulses/day of 2 h duration. Intrahepatic infusion of oPRL was not more effective than intrajugular delivery regardless of the schedule of administration. These results indicate that pulse duration is a more important determinant of the effectiveness of the galactopoietic action of PRL in the lactating rat than is pulse frequency. No evidence was obtained that the liver participates in the galactopoietic effects of PRL.