MOLECULAR AGGREGATION CHARACTERIZED BY HIGH-ORDER AUTOCORRELATION IN FLUORESCENCE CORRELATION SPECTROSCOPY
MOLECULAR AGGREGATION CHARACTERIZED BY HIGH-ORDER AUTOCORRELATION IN FLUORESCENCE CORRELATION SPECTROSCOPY
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DOI：
10.1016/s0006-3495(87)83213-7
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发表时间：
1987-08-01
影响因子：
3.4
通讯作者：
THOMPSON, NL
中科院分区：
文献类型：
--
作者：
PALMER, AG;THOMPSON, NL
描述了在荧光相关光谱中使用高阶自相关来研究包含荧光分子的样品中的聚集。推导了荧光涨落自相关函数gm，n（τ）= [（Δ fm（t + τ）Δ fm（t）-（Δ Fm（t）（Δ Fn（t））]/（F）m+n的理论表达式，其中Δ F（t）是时间t的荧光涨落，（F）是平均荧光，m和n是小于或等于3的整数。概述了从一系列Gm，n（0）值确定不同尺寸聚集体的数密度和相对荧光产率的方法。方法适用于1，1 '-双十八烷基-3，3，3'，3 '-四甲基吲哚羰花青高氯酸盐在水和乙醇中的悬浮液。所提出的技术可能被证明是有用的荧光标记的生物分子，如细胞表面受体的检测和表征的聚集体。
The use of high order autocorrelation in fluorescence correlation spectroscopy for investigating aggregation in a sample that contains fluorescent molecules is described. Theoretical expressions for the fluorescence fluctuation autocorrelation functions defined by gm,n(tau) = [(delta fm(t + tau)delta fm(t] - (delta Fm(t] (delta Fn(t]]/(F)m+n, where delta F(t) is the fluorescence fluctuation at time t, (F) is the average fluorescence, and m and n are integers less than or equal to 3, are derived. Methods for determining the number densities and relative fluorescence yields of aggregates of different sizes from a series of Gm,n(0) values are outlined. The method is applied to 1,1'-dioctadecyl-3,3,3',3'-tetramethylindocarbocyanine perchlorate suspended in solutions of water and ethyl alcohol. The technique presented may prove useful in detecting and characterizing aggregates of fluorescent-labeled biological molecules such as cell surface receptors.