Recurrent episodes of gram-negative bacteremia or endotoxemia change reactivity of pre- and post-capillary pulmonary segments to angiotensin or free radicals.
Recurrent episodes of gram-negative bacteremia or endotoxemia change reactivity of pre- and post-capillary pulmonary segments to angiotensin or free radicals.
复制标题
革兰氏阴性菌血症或内毒素血症的反复发作改变了毛细血管前和后肺段对血管紧张素或自由基的反应性。
DOI：
10.1007/bf01706478
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发表时间：
1992
影响因子：
38.9
通讯作者：
Jones,RC
中科院分区：
文献类型：
--
作者：
Kirton,OC;Gore,RG;Reid,LM;Jones,RC
目的：反复发作的革兰氏阴性菌血症(腹膜脓肿)或内毒素血症可导致大鼠肺微血管损伤。设计：在离体肺制备中，阻力分为毛细血管前段(PVRa)和毛细血管后段(PRVv)：血管反应性通过血管紧张素II(AII)或活性氧代谢物的激发来评估。动物每周接受4次LiveE腹内植入。科兰德B。Fragilisin，无菌盲肠内容物和硫酸钡载体(败血症)或单独载体(假脓毒症)：或每周4次静脉滴注E。结肠内毒素(Endo)或生理盐水(Sham Endo)。第五组为未经处理的对照组(对照组)。测量和结果：在脓毒症和内脏肺中，激发前PVRa和PVRv正常。在脓毒症肺中，AII使PVRa增加的幅度大于对照肺，而PVRv在两种情况下的增加程度相似。在内脏肺中，它增加了PVRa，与其在假手术中的作用相比：在两者中，它也增加了PVRv，程度相似，但远高于基线水平。随着剂量的增加(分别达到25μm和50μm)，总是会出现快速反应。氧自由基致脓毒症大鼠肺内膜和肺内膜明显收缩，尤其是PVRv，而对照组和假手术组肺血管无明显反应。结论：脓毒症和内毒素后肺血管反应性异常明显，两种药物均可检测到局部特异性改变。
Objective: Recurrent episodes of Gram-negative bacteremia (from intraperitoneal abscesses) or endotoxemia cause lung microvascular injury in the rat. Change in vascular reactivity was assessed in response to challenge.Design: In the isolated lung preparation, resistance was partitioned between pre-(PVRa) and post-capillary (PRVv) segments: vasoreactivity was assessed by challenge with Angiotensin II (AII) or reactive oxygen metabolites. Animals received 4 weekly intra-abdominal implants of liveE. coliandB. fragilisin a carrier of sterile cecal content and barium sulfate (SEPSIS) or carrier alone (SHAM SEPSIS): or 4 weekly intravenous infusion ofE. coliendotoxin (ENDO) or of saline (SHAM ENDO). A fifth group were untreated controls (CONTROL).Measurements and results:In the SEPSIS and ENDO lungs, PVRa and PVRv before challenge were normal. In the SEPSIS lung, AII increased PVRa more than in the control lung, PVRv to a similar degree in both. In the ENDO lung it increased PVRa compared with its effect on the SHAM ENDO lung: In both it also increased PVRv, to a similar degree and well above the baseline. Always tachyphylaxis developed with increases in dosage (to 25 μm and 50 μm, respectively). Oxygen free radical challenge in the SEPSIS and ENDO lung caused significant vasoconstriction, particularly PVRv, whereas no response was observed in the CONTROL or SHAM-treated lung from either group.Conclusion: Abnormal lung vascular reativity after SEPSIS or ENDOTOXIN is evident on challenge, the two agents used here detecting site specific changes.