Rice ubiquitin promoters: deletion analysis and potential usefulness in plant transformation systems
Rice ubiquitin promoters: deletion analysis and potential usefulness in plant transformation systems
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DOI：
10.1007/s00299-003-0657-y
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发表时间：
2003-09-01
影响因子：
6.2
通讯作者：
Oard, JH
中科院分区：
文献类型：
--
作者：
Wang, J;Oard, JH
强的组成型启动子形成了使用转基因植物进行基础和应用研究的基石。在转基因水稻[Oryza sativa（L.）]中，来自含有RUBQ 1或RUB 2水稻泛素启动子的构建体的GUS（β-葡萄糖醛酸酶）表达水平高8至35倍。与35 S启动子相比，对RUBQ 2的5 ′-上游区域的缺失分析揭示了一个推定的增强子区域，其产生瞬时GUS表达的2.4倍增加。Southern杂交分析表明，3 ~ 7个拷贝的GUS基因稳定地插入到R 0和R1植株中，并以单基因方式遗传。
Strong constitutive promoters form a cornerstone for basic and applied research using transgenic plants. GUS (beta-glucuronidase) expression levels from constructs containing RUBQ1 or RUB2 rice ubiquitin promoters were 8- to 35-fold higher in transgenic rice [Oryza sativa (L.)] plants, respectively, when compared to the 35S promoter. Deletion analysis of the 5'-upstream region of RUBQ2 revealed a putative enhancer region that produced a 2.4-fold increase in transient GUS expression. Southern blot analysis showed that three to seven copies of the GUS gene were stably inserted into R0 and R1 plants and inherited in a monogenic fashion.