XYLOGLUCAN AND BETA-D-GLUCAN IN CELL-WALLS OF RICE SEEDLINGS
XYLOGLUCAN AND BETA-D-GLUCAN IN CELL-WALLS OF RICE SEEDLINGS
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DOI：
10.1093/oxfordjournals.pcp.a076357
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发表时间：
1982-01-01
影响因子：
4.9
通讯作者：
MATSUDA, K
中科院分区：
文献类型：
--
作者：
KATO, Y;ITO, S;MATSUDA, K
用草酸铵-草酸铵、4%KOH和24%KOH连续提取4日龄水稻幼苗的细胞壁。分别从4%KOH提取物和24%KOH提取物中分离得到β-D-葡聚糖制剂和木糖葡聚糖制剂。甲基化分析和酶降解研究表明，前者主要是由3-O-β-纤维二糖-D-葡萄糖和3-O-β-纤维三糖-D-葡萄糖组成的重复低聚糖单元，摩尔比为2.6：1.0，后者是由α-D-木糖基-(1.fwdarw)重复低聚糖单元组成。6)-β-D-葡萄糖基-(1.fwdarw.4)-[α-D-木糖基-(1.fwdarw.6)-β-D-葡萄糖基-(1.fwdarw.4)-D-葡萄糖，α-D-木糖基-(1.fwdarw.6)-β-D-葡萄糖基-(1.fwdarw.4)-D-葡萄糖和纤维二糖。
Cell walls of 4 day old rice seedlings were extracted successively with ammonium oxalate-oxalic acid, 4% KOH and 24% KOH. A .beta.-D-glucan preparation and a xyloglucan preparation were isolated from the 4% KOH extract and 24% KOH extract, respectively. Methylation analysis and enzymic degradation studies of the polysaccharides showed that the former was built up predominantly of repeating-oligosaccharide units of 3-O-.beta.-cellobiosyl-D-glucose and 3-O-.beta.-cellotriosyl-D-glucose in a molar ratio of 2.6:1.0; the latter was of repeating oligosaccharide units of .alpha.-D-xylosyl-(1 .fwdarw. 6)-.beta.-D-glucosyl-(1 .fwdarw. 4)-[.alpha.-D-xylosyl-(1 .fwdarw. 6)]-.beta.-D-glucosyl-(1 .fwdarw. 4)-D-glucose, .alpha.-D-xylosyl-(1 .fwdarw. 6)-.beta.-D-glucosyl-(1 .fwdarw. 4)-D-glucose and cellobiose.