Comparative study of cadmium effects on membrane lipid composition of Brassica juncea and Brassica napus leaves
Comparative study of cadmium effects on membrane lipid composition of Brassica juncea and Brassica napus leaves
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DOI：
10.1016/j.plantsci.2005.10.003
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发表时间：
2006-03-01
期刊：
影响因子：
5.2
通讯作者：
Zarrouk, M
中科院分区：
文献类型：
--
作者：
Nouairi, I;Ben Ammar, W;Zarrouk, M
植物修复是用来描述植物从污染土壤中清除重金属的过程。在本研究中，我们研究了甘蓝型油菜对Cd的积累，并与已知的Cd超积累植物芥菜进行了比较。在水培介质中。Cd处理以0~50亩M的浓度系列施入15天。两种植物吸收的Cd大部分保留在根中，但芥菜比油菜在地上部积累了更多的Cd。过量的金属供应导致镉处理下芥菜叶片的脂肪含量增加，但不影响其脂肪酸组成。相反，甘蓝型油菜叶片的脂类成分发生了变化，脂类含量也有所下降。单半乳糖二酰甘油(MGDG)、双半乳糖二甘油(DGDG)、磺基脂肪(SL)和磷脂酰甘油(PG)的含量在金属处理下显著降低。而叶外脂质：磷脂酰胆碱(PC)和磷脂酰乙醇胺(PE)的含量显著增加。后者的发现表明，金属的应用促进了PC和PE的合成。PG的多不饱和脂肪酸主要是亚麻酸(C18：3)和十六碳三烯酸(C16：3)，反式棕榈油酸(C16：1T)含量下降。因此，镉似乎优先影响含有较高水平的多不饱和脂肪酸的氯化塑料脂类。与Cd敏感种甘蓝型油菜相比，Cd超积累植物芥菜型油菜的细胞膜对Cd胁迫的稳定性更强。(C)2005爱思唯尔爱尔兰有限公司。保留所有权利。
The term phytoremediation is used to describe the clean-up of heavy metals from contaminated soils by plants. In this study, we examined cadmium (Cd) accumulation by Brassica napus in comparison with the known Cd-hyperaccumulator Brassica juncea. in a hydroponic medium. Cd treatment was applied as a concentration series between 0 and 50 mu M for 15 days. Most of the Cd taken up was retained in roots in both species, however, B. juncea accumulated more Cd in the shoots compared to B. napus. Excess metal supply resulted in an increase in the lipid content of B. juncea leaves grown under cadmium treatment, but did not affect fatty acids composition. In contrast, an alteration in the lipid composition of B. napus leaves was observed together with a decrease in the lipid contents. The amounts of chloroplastic lipids: monogalactosyldiacylglycerol (MGDG), digalactosyldiacylglycerol (DGDG), sulfolipids (SL) and phosphatidylglycerol (PG) decreased drastically under the effect of metallic treatments. Whereas, amounts of extrachloroplastic lipids: phosphatidylcholine (PC) and phosphatidylethanolamine (PE) were significantly increased. The latter finding suggested that PC and PE synthesis was enhanced by metal application. Moreover, the levels of polyunsaturated fatty acids mainly linolenic acid (C18:3) and hexadecatrienoic acid (C16:3) and that of trans palmitoleic acid (C16:1t) from PG declined. So, cadmium seems to affect preferentially chloroplastic lipids containing higher levels of polyunsaturated fatty acids. Lipid changes in B. juncea, the well known Cd-hyperaccumulator specie, revealed a more stability of its cellular membranes to cadmium-stress as compared to Cd-sensitive specie, B. napus. (c) 2005 Elsevier Ireland Ltd. All rights reserved.