Significantly improved production of Welan gum by Sphingomonas sp. WG through a novel quorum-sensing-interfering dipeptide cyclo (L-Pro-L-Phe)
Significantly improved production of Welan gum by Sphingomonas sp. WG through a novel quorum-sensing-interfering dipeptide cyclo (L-Pro-L-Phe)
复制标题
鞘氨醇单胞菌显着提高了韦兰胶的产量。
DOI：
10.1016/j.ijbiomac.2018.12.189
复制
发表时间：
2019
影响因子：
8.2
通讯作者：
Shen Ya-ling
中科院分区：
文献类型：
--
作者：
Zhu Hu;Sun Shi-wei;Li Hui;Chang Aiping;Liu Yu-chen;Qian Jin;Shen Ya-ling
微生物中的胞外多糖合成、抗生素产生和初级代谢等多种生理功能都受到群体感应的严格控制。在这项研究中，一种来自海洋的细菌鞘氨醇单胞菌。 WG被发现具有环(L-Pro-L-Phe)介导的群体感应机制。由鞘氨醇单胞菌产生的环（L-Pro-L-Phe）。 WG 在该群体感应系统中充当信号分子。这是首次尝试将环状二肽表征为鞘氨醇单胞菌中的群体感应信号分子。该结果有效地使经典群体感应理论更加完善。此外，补充分离的环(L-Pro-L-Phe)导致鞘氨醇单胞菌分泌的韦兰胶产量增加15%。 WG在深层发酵中。本研究提供的数据将为探索环状二肽在调节韦兰胶生产中的作用提供证据。
A variety of physiological functions such as exopolysaccharide synthesis, antibiotic production, and primary metabolism are tightly controlled by quorum sensing in microorganisms. In this study, a marine-derived bacteriumSphingomonassp. WG was found to possess a cyclo(L-Pro-L-Phe)-mediated quorum sensing mechanism. The cyclo(L-Pro-L-Phe) produced bySphingomonassp. WG functions as a signalling molecule in this quorum sensing system. It is the first attempt to characterize cyclic dipeptides as quorum sensing signalling molecules inSphingomonassp. and the results effectively make the classical quorum sensing theory more perfect. Furthermore, the supplementation with isolated cyclo(L-Pro-L-Phe) resulted in a 15% increase in the production of welan gum secreted bySphingomonassp. WG in the submerged fermentation. The data presented in this study will provide evidences for exploring the role of cyclic dipeptides in regulating the production of welan gum.