The electrochemical behavior and amperometric determination of tyrosine and tryptophan at a glassy carbon electrode modified with butyrylcholine
The electrochemical behavior and amperometric determination of tyrosine and tryptophan at a glassy carbon electrode modified with butyrylcholine
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DOI：
10.1016/j.elecom.2004.03.006
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发表时间：
2004-05-01
影响因子：
5.4
通讯作者：
Lin, XQ
中科院分区：
文献类型：
--
作者：
Jin, GP;Lin, XQ
首先采用电化学方法制备了单层丁酰胆碱（BuCh）修饰玻碳电极（BuCh/GCE）。 X射线光电子能谱、原位紫外-可见光谱电化学和电化学技术用于表征电极表面。结果表明，BuCh 在电化学种植过程中被水解为胆碱 (Ch) 和丁酸。 Ch和丁酸产物均固定在碳表面，形成共价种植的Ch单层，通过特殊的吸附机制将丁酸固定在单层中。这提供了双组分数字混合单层修饰电极的新颖示例。 BuCh/GCE 的电化学活性主要针对氨基酸的电化学催化进行了测试。研究发现，酪氨酸（Tyr）和色氨酸（Trp）可以在该电极上被催化氧化，并具有扩散控制特性，可应用于实际样品的分析灵敏度。在静态溶液中，电极处的差分脉冲伏安峰值电流的线性范围为4 x 10(-6)-1 x 10(-4) M Tyr和2 x 10(-6)-6 x 10(-5) M Trp，检测限分别为4 x 10(-7) M Tyr和6 x 10(-7) M Trp。 BuCh/GCE 传感器在至少一个月的运行中保持稳定且可重复。 BuCh/GCE 的电化学活性与类似制备的 Ch、乙酰胆碱和丙酰胆碱修饰电极进行了比较。 (C) 2004 Elsevier B.V. 保留所有权利。
A monolayer butyrylcholine (BuCh) modified glassy carbon electrode (BuCh/GCE) was first prepared by electrochemical method. X-ray photoelectron spectroscopy, in situ UV-Vis spetroelectrochemistry, and electrochemical techniques were used for characterization of the electrode surface. It was demonstrated that the BuCh was hydrolyzed to choline (Ch) and butyric acid during the electrochemical planting process. Both Ch and butyric acid products were immobilized onto the carbon surface forming a covalently planted Ch monolayer, which immobilized the butyric acid in the monolayer by a special adsorption mechanism. This provided a novel example of two-component digitally mingled monolayer modified electrode. The electrochemical activity of the BuCh/GCE was primarily tested for electrochemical catalyses of amino acids. It was found that tyrosine (Tyr) and tryptophan (Trp) could be catalytically oxidized with diffusion-controlled characteristics at this electrode, which could be applied for analytical sensitivity in practical sample. In static solutions, the linear ranges of the differential pulse voltammetry peak current at the electrode were 4 x 10(-6)-1 x 10(-4) M Tyr and 2 x 10(-6)-6 x 10(-5) M Trp with detection limits of 4 x 10(-7) M Tyr and 6 x 10(-7) M Trp, respectively. The BuCh/GCE sensor was stabile and reproducible for at least month operation. The electrochemical activity of the BuCh/GCE was compared with similarly prepared Ch, acetylcholine and propionylcholine-modified electrodes. (C) 2004 Elsevier B.V. All rights reserved.