Oral immunization with transgenic rice seeds expressing VP2 protein of infectious bursal disease virus induces protective immune responses in chickens.
Oral immunization with transgenic rice seeds expressing VP2 protein of infectious bursal disease virus induces protective immune responses in chickens.
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DOI：
10.1111/j.1467-7652.2007.00270.x
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发表时间：
2007-09
影响因子：
13.8
通讯作者：
Jian-xiang Wu;Lian Yu;Long Li;Jinqiang Hu;Jiyong Zhou;Xue-ping Zhou
中科院分区：
文献类型：
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作者：
Jian-xiang Wu;Lian Yu;Long Li;Jinqiang Hu;Jiyong Zhou;Xue-ping Zhou
研究了传染性法氏囊病病毒（IBDV）宿主保护性免疫原VP 2蛋白在水稻中的表达及其对鸡的免疫原性和保护力。将传染性法氏囊病病毒ZJ 2000株的VP 2基因克隆到水稻谷蛋白GluA-2基因的Gt 1启动子下游，构建成双元表达载体pCambia 1301-Gt 1。利用农杆菌介导法转化水稻胚性愈伤组织，获得了121个转基因株系，并在温室中生长成熟。VP 2蛋白在转基因水稻种子中的表达量为可溶性蛋白的0.678%~ 4.521%（μ g/mg）。用表达VP 2蛋白的转基因水稻种子经口接种特定病原体的鸡产生针对IBDV的中和抗体，并且当用高毒力IBDV毒株BC 6/85攻击时受到保护。这些结果表明，表达IBDV VP 2的转基因水稻种子可用作有效、安全和廉价的IBDV疫苗。
The expression of infectious bursal disease virus (IBDV) host-protective immunogen VP2 protein in rice seeds, its immunogenicity and protective capability in chickens were investigated. The VP2 cDNA of IBDV strain ZJ2000 was cloned downstream of the Gt1 promoter of the rice glutelin GluA-2 gene in the binary expression vector, pCambia1301-Gt1. Agrobacterium tumefaciens containing the recombinant vector was used to transform rice embryogenic calli, and 121 transgenic lines were obtained and grown to maturity in a greenhouse. The expression level of VP2 protein in transgenic rice seeds varied from 0.678% to 4.521% microg/mg of the total soluble seed protein. Specific pathogen-free chickens orally vaccinated with transgenic rice seeds expressing VP2 protein produced neutralizing antibodies against IBDV and were protected when challenged with a highly virulent IBDV strain, BC6/85. These results demonstrate that transgenic rice seeds expressing IBDV VP2 can be used as an effective, safe and inexpensive vaccine against IBDV.