Gelsolin and ADF/cofilin enhance the actin dynamics of motile cells.
Gelsolin and ADF/cofilin enhance the actin dynamics of motile cells.
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DOI：
10.1073/pnas.97.13.6936
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发表时间：
2000-06
影响因子：
11.1
通讯作者：
F. Southwick
中科院分区：
文献类型：
--
作者：
F. Southwick
Motile nonmuscle cells rapidly remodel their actin cytoskeleton to change shape as they crawl. The mechanisms underlying these dynamic changes in actin-filament concentration, length, and architecture have been the subject of extensive study for nearly three decades. Ameboid movements are complex and multidirectional, making analysis of individual cells difficult. Therefore investigators have tried to simplify their experimental models by (i) purifying individual proteins and analyzing their interactions with actin; (ii) studying the behavior of actin in cytoplasmic extracts. This condition allows the introduction of various inhibitors and stimulators and the addition and subtraction of individual proteins; it also allows the introduction of Listeria monocytogenes and Shigella flexneri, intracellular parasites that form discrete polymerization zones to propel them through the host cell cytoplasm; (iii) microinjecting individual proteins and inhibitory peptides into intact cells or transfecting cells with either sense and antisense cDNA; and (iv) knocking out individual genes. Each approach has advantages and disadvantages that need to be considered in interpreting results and assessing the contribution of individual proteins to the complex orchestration of changes required for actin-based motility. The temptation has been to oversimplify actin-based motility and tout an individual protein as being of primary importance to the exclusion of others. Certain proteins become fashionable and tend to dominate the literature. However, investigators need to maintain an open-minded perspective that is inclusive rather than exclusive.
