Altered position of cell bodies and fibers in the ventromedial region in SF-1 knockout mice
Altered position of cell bodies and fibers in the ventromedial region in SF-1 knockout mice
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DOI：
10.1016/j.expneurol.2011.08.021
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发表时间：
2011-12-01
影响因子：
5.3
通讯作者：
Majdic, Gregor
中科院分区：
文献类型：
--
作者：
Buedefeld, Tomaz;Tobet, Stuart A.;Majdic, Gregor
下丘脑腹内侧核（VMH）是下丘脑内侧-基底核中参与能量平衡调节的关键细胞群。先前的研究表明，VMH的细胞组织在类固醇生成因子-1（NR 5a 1）基因破坏的小鼠（SF-1 KO小鼠）中发生改变。本研究检查食欲/食欲肽（神经肽Y（NPY），刺豚鼠相关肽（AgRP）和可卡因和安非他明调节的转录（CART））和神经连接，并从SF 1基因敲除小鼠的VMH。采用NeuroVue示踪法和Golgi染色法分别观察视前区（POA）与VMH之间的联系以及VMH内树突的方向。这项研究的结果揭示了VMH区域的细胞结构的变化与免疫反应性神经肽Y，银蛋白和CART的分布。在WT小鼠中，来自POA的投射通常围绕VMH，而在SF-1 KO小鼠中，来自POA的投射流过否则将是VMH的区域。高尔基体浸渍的区域显示较少的树突与腹外侧的方向和一般情况下，更可变的树突的方向在SF-1基因敲除小鼠提供额外的证据表明，该地区的细胞的连接性可能会改变，由于细胞重排随之而来的破坏NR 5a 1基因。总之，这项研究极大地扩展了数据显示，在SF-1 KO小鼠中，含有VMH的区域的形态被破坏，并表明含有NPY、AgRP和CART的细胞或纤维的位置的变化可能部分地解释了这些小鼠体重稳态的变化。(C)2011 Elsevier Inc. All rights reserved.
The ventromedial nucleus of the hypothalamus (VMH) is a key cell group in the medial-basal hypothalamus that participates in the regulation of energy balance. Previous studies have shown that the cellular organization of the VMH is altered in mice with a disruption of the steroidogenic factor-1 (NR5a1) gene (SF-1 KO mice). The present study examined orexigenic/anorexigenic peptides (neuropeptide Y (NPY), agouti-related peptide (AgRP) and cocaine- and amphetamine-regulated transcript (CART)) and neural connections to and from the VMH in SF1 KO mice. NeuroVue tracing and Golgi staining were used to evaluate connections between the preoptic area (POA) and VMH and the orientation of dendrites in the VMH, respectively. Results of this study reveal changes in the cytoarchitecture of the region of the VMH with respect to the distribution of immunoreactive NPY, AgRP and CART. In WT mice projections from the POA normally surround the VMH while in SF-1 KO mice, projections from the POA stream through the region that would otherwise be VMH. Golgi impregnation of the region revealed fewer dendrites with ventrolateral orientations and in general, more variable dendritic orientations in SF-1 KO mice providing additional evidence that the connectivity of cells in the region is likely altered due to the cellular rearrangements consequent to disruption of the NR5a1 gene. In conclusion, this study greatly extends the data showing that the morphology of the regions containing the VMH is disrupted in SF-1 KO mice and suggests that changes in the location of cells or fibers containing NPY, AgRP and CART may, in part, account for changes in body weight homeostasis in these mice. (C) 2011 Elsevier Inc. All rights reserved.