A Microfluidic Device for Hydrodynamic Trapping and Manipulation Platform of a Single Biological Cell
A Microfluidic Device for Hydrodynamic Trapping and Manipulation Platform of a Single Biological Cell
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DOI：
10.3390/app6020040
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发表时间：
2016-02-01
影响因子：
2.7
通讯作者：
Zulkifli, Razauden Mohamed
中科院分区：
文献类型：
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作者：
Khalili, Amelia Ahmad;Ahmad, Mohd Ridzuan;Zulkifli, Razauden Mohamed
为了对单个细胞进行特异性分析，在进行进一步的处理和分析之前，必须捕获单个细胞并将其相互分离。本文介绍了一种基于流体动力学技术的捕获单个细胞/颗粒的微流体装置的设计、模拟、制造和测试。一种t通道捕获芯片已被提出提供单细胞捕获，因此可以作为细胞处理和操作的平台。利用Abaqus有限元分析软件建立了t型通道的有限元捕获模型，通过优化通道的几何形状和Rh-Main/Rh-Trap比值，观察其捕获能力。利用HUVEC细胞聚集体进行了模拟分析和实验，验证了t通道模型中细胞捕获的概念。通过使用适当的通道几何形状和Rh-Main/Rh-Trap比率，发现t通道能够通过水动力捕获概念捕获单个细胞。所提出的t通道单细胞捕获在单细胞表征和单细胞三维聚集体处理和分析方面具有潜在的应用前景。
To perform specific analysis for the single cell, individual cells have to be captured and separated from each other before further treatments and analysis can be carried out. This paper presents the design, simulation, fabrication, and testing of a microfluidic device for trapping a single cell/particle based on a hydrodynamic technique. A T-channel trapping chip has been proposed to provide single-cell trapping and consequently could be a platform for cell treatments and manipulations. A finite element T-channel trapping model was developed using Abaqus FEA software to observe it's trapping ability by optimizing the channel's geometry and Rh-Main/Rh-Trap ratio. A proof of concept demonstration for cell trapping in the T-channel model was presented in the simulation analysis and experimental work using HUVEC cell aggregate. The T-channel was found to be able to trap a single cell via the hydrodynamic trapping concept using an appropriate channel geometry and Rh-Main/Rh-Trap ratio. The proposed T-channel single-cell trapping has potential application for single cell characterization and single 3D cell aggregates treatments and analysis.