Encapsulation of lycopene in Chlorella pyrenoidosa: Loading properties and stability improvement
Encapsulation of lycopene in Chlorella pyrenoidosa: Loading properties and stability improvement
复制标题
蛋白核小球藻中番茄红素的封装：负载特性和稳定性改进
DOI：
10.1016/j.foodchem.2017.05.069
复制
发表时间：
2017-11-15
期刊：
影响因子：
8.8
通讯作者：
Tang, Wenting
中科院分区：
文献类型：
--
作者：
Pu, Chuanfen;Tang, Wenting
为了提高番茄红素的稳定性并将其整合到复杂的营养保健品中，开发了外源性番茄红素负载小球藻（Chlorella pyrenoidosa, CPCs）细胞。该配合物包封率为13.06 +/- 0.89%，包封效率为96.31 +/- 3.10%。荧光分析表明，番茄红素被包裹在CPCs中。通过x射线衍射、热重和差示扫描量热分析，对未负载的CPCs、番茄红素及其物理混合物进行了比较。这些研究表明，番茄红素在复合物中是无定形的。降解动力学表明，包封提高了番茄红素的稳定性。经25℃贮藏25 d后，番茄红素负载的CPCs对DPPH自由基的抗氧化活性高于未包封的CPCs。本研究证明了将番茄红素包封在CPCs内的可行性，并将两种材料的活性结合起来，可用于新型营养保健品的生产，以降低氧化应激。(C) 2017 Elsevier Ltd.版权所有。
Aiming to improve the stability of lycopene and incorporate it into a complex nutraceutical, exogenous lycopene-loaded Chlorella pyrenoidosa cells (CPCs) were developed. The complex had an encapsulation yield of 13.06 +/- 0.89% and an encapsulation efficiency of 96.31 +/- 3.10%. Fluorescence analyses indicated that lycopene was encapsulated in the CPCs. X-ray diffraction, thermogravimetric and differential scanning calorimetric analyses were conducted and compared to those of the non-loaded CPCs, lycopene and their physical mixture. These studies demonstrated that lycopene was amorphous in the complex. The degradation kinetics indicated that encapsulation increased the stability of lycopene. The antioxidant activity of lycopene loaded CPCs against DPPH free radicals was higher than that of the unencapsulated lycopene after storage at 25 degrees C for 25 d. This study proved the feasibility of encapsulation of lycopene in the CPCs and combined the activities of both materials, which could be employed in the production of novel nutraceuticals to reduce oxidative stress. (C) 2017 Elsevier Ltd. All rights reserved.