Detection and characterization of receptor interactions with PDZ domains.
Detection and characterization of receptor interactions with PDZ domains.
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受体与 PDZ 结构域相互作用的检测和表征。
DOI：
10.1007/978-1-61779-160-4_21
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发表时间：
2011
期刊：
影响因子：
--
通讯作者：
Hall,RandyA
中科院分区：
文献类型：
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作者：
Ritter,StefanieL;Hall,RandyA
许多跨膜受体受含有PDZ结构域的支架蛋白的调节，这些支架蛋白与受体羧基末端相互作用，控制受体的信号、运输和定位。我们在这里描述了检测和表征受体和PDZ支架之间相互作用的方法。这些方法包括构建和筛选蛋白质组阵列，使用融合蛋白的印迹覆盖，以及评估细胞内相互作用的免疫共沉淀研究。
Many transmembrane receptors are regulated by associations with scaffold proteins containing PDZ domains, which interact with receptor carboxyl-termini to control receptor signaling, trafficking, and localization. We describe here approaches for detecting and characterizing interactions between receptors and PDZ scaffolds. These approaches include the construction and screening of proteomic arrays, blot overlays using fusion proteins, and co-immunoprecipitation studies to assess interactions in cells.