Expression of the G protein γT1 subunit during zebrafish development
Expression of the G protein γT1 subunit during zebrafish development
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DOI：
10.1016/j.modgep.2007.01.003
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发表时间：
2007-04-01
影响因子：
1.2
通讯作者：
Robishaw, Janet D.
中科院分区：
文献类型：
--
作者：
Chen, Hui;Leung, TinChung;Robishaw, Janet D.
本文报道了斑马鱼发育过程中G蛋白γ T1亚基基因（gngT1）的鉴定和表达分析。与人类和小鼠的同源物相似，我们证实斑马鱼的gngT1在发育中的视网膜中表达，其转录与光受体细胞特异性标记物视紫红质（rho）重叠。令人惊讶的是，我们还发现斑马鱼gngT1在背间脑中表达，其转录与松果体特异性标记，芳基烷基胺n -乙酰转移酶-2 （annat-2）和眼外视紫红质（exorh）重叠。对斑马鱼gngT1基因近端启动子序列的分析发现了转录因子的锥杆同源盒/正畸（Crx/Otx）同源结构域家族的几个保守结合位点。利用斑马鱼的morpholino反义方法，我们发现靶向敲低otx5能有效抑制松果体中gngT1的表达，而敲低crx则能显著降低视网膜中gngT1的表达。综上所述，这些数据表明gngT1基因的松果体和视网膜特异性表达受到不同转录因子和外源信号的控制。(c) 2007 Elsevier B.V.版权所有
Here, we report the identification and expression analysis of the zebrafish G protein gamma T1 subunit gene (gngT1) during development. Similar to its human and mouse homologs, we confirm zebrafish gngT1 is expressed in the developing retina, where its transcription overlaps with the photoreceptor cell-specific marker, rhodopsin (rho). Surprisingly, we also show zebrafish gngT1 is expressed in the dorsal diencephalon, where its transcription overlaps with the pineal specific markers, arylalkylamine N-acetyltransferase-2 (annat-2) and extraocular rhodopsin (exorh). Analysis of the proximal promoter sequence of the zebrafish gngT1 gene identifies several conserved binding sites for the cone-rod homeobox/orthodenticle (Crx/Otx) homeodomain family of transcription factors. Using a morpholino anti-sense approach in zebrafish, we show that targeted knockdown of otx5 potently suppresses gngT1 expression in the pineal gland, whereas knockdown of crx markedly reduces gngT1 expression in the retina. Taken together, these data indicate that pineal- and retinal-specific expression of the gngT1 gene are controlled by different transcription factors and exogenous signals. (c) 2007 Elsevier B.V. All rights reserved.