Characterization of wheat miRNAs and their target genes responsive to cadmium stress
Characterization of wheat miRNAs and their target genes responsive to cadmium stress
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小麦 miRNA 及其靶基因对镉胁迫响应的表征
DOI：
10.1016/j.plaphy.2016.01.020
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发表时间：
2016-04-01
影响因子：
6.5
通讯作者：
Zhang, Liang
中科院分区：
文献类型：
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作者：
Qiu, ZongBo;Hai, BenZhai;Zhang, Liang
越来越多的microRNA已被证明在植物对各种金属胁迫的响应中发挥重要的调节作用。然而，关于小麦中的miRNAs，特别是对镉（Cd）胁迫响应的miRNAs的研究却很少。为了研究miRNAs在镉胁迫响应中的作用，以小麦幼苗为材料，研究了250 μ M镉胁迫6、12、24和48 h后小麦幼苗的形态和生理变化以及5种miRNAs及其靶基因的表达。我们的研究结果表明，miRNA及其靶基因在镉胁迫下小麦幼苗的叶和根中差异表达。此外，miR398可能通过调节其靶点CSD参与Cd胁迫，从而参与氧化胁迫耐受。在所研究的10对miRNA靶点中，有9对在Cd胁迫下小麦幼苗的叶片和根系中表现出复杂的调控关系。这些结果表明，miRNAs参与了小麦Cd胁迫信号转导的调控。对miRNAs及其相关靶基因的研究为理解植物重金属耐性的分子机制提供了一个框架。(C)2016 Elsevier Masson SAS。All rights reserved.
A increasing number of microRNAs have been shown to play important regulatory roles in plant responses to various metal stresses. However, little information about miRNAs especially miRNAs responsive to cadmium (Cd) stress is available in wheat. To investigate the role of miRNAs in responses to Cd stress, wheat seedlings were subjected to 250 mu M Cd solution for 6, 12, 24 and 48 h, and analyses of morphological and physiological changes as well as the expression of five miRNAs and their corresponding targets were carried out. Our results demonstrated that miRNAs and their targets were differentially expressed in leaves and roots of wheat seedlings exposed to Cd stress. Furthermore, miR398 may involve in oxidative stress tolerance by regulating its target CSD to participate in Cd stress. Among ten miRNA-target pairs studied, nine pairs showed complex regulation relationship in leaves and roots of wheat seedlings exposed to Cd stress. These findings suggested that miRNAs are involved in the mediation of Cd stress signaling responses in wheat. The characterization of the miRNAs and the associated targets in responses to Cd exposure provides a framework for understanding the molecular mechanism of heavy metal tolerance in plants. (C) 2016 Elsevier Masson SAS. All rights reserved.