Deletion of the c-Ha-ras-1 allele in human skin cancers.
Deletion of the c-Ha-ras-1 allele in human skin cancers.
复制标题
人类皮肤癌中 c-Ha-ras-1 等位基因的缺失。
DOI：
10.1002/mc.2940020510
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发表时间：
1989
影响因子：
4.6
通讯作者：
Bales,ES
中科院分区：
文献类型：
--
作者：
Ananthaswamy,HN;Applegate,LA;Goldberg,LH;Bales,ES
先前的研究表明，位于11号染色体短臂上的c-Ha-ras-1和其他基因在许多人类肿瘤中经常丢失。我们研究了c-Ha-ras-1等位基因的类似丢失是否发生在人类鳞状细胞癌（SCC）和基底细胞癌（BCC）中。从35对皮肤肿瘤（25个BCC和10个SCC）和来自相同患者的匹配正常皮肤中分离DNA，并通过Southern印迹杂交分析c-Ha-ras基因多态性。16名BCC患者和1名SCC患者在其正常皮肤DNA中的c-Ha-ras基因为组成性杂合。在这17例患者中，5例患者（4例BCC和1例SCC）（29%）显示肿瘤DNA中的一个c-Ha-ras等位基因缺失。其中一名体质性杂合子BCC患者表现出6.6-kb c-Ha-ras等位基因的缺失和7.8-kb等位基因的额外拷贝。总之，在研究的17例人类皮肤癌中，c-Ha-ras基因座的杂合性丢失频繁发生（29%）。然而，我们发现90%的SCC患者，而不是36%的BCC患者，在正常皮肤组织中只有一个c-Ha-ras等位基因，需要进一步研究。c-Ha-rashomozygosity是否与SCC的遗传易感性有关仍有待确定。
Previous studies have shown that c‐Ha‐ras‐1 and other genes located on the short arm of chromosome 11 are frequently lost in a number of human tumors. We investigated whether similar losses of the c‐Ha‐ras‐1 allele occurred in human squamous cell carcinomas (SCCs) and basal cell carcinomas (BCCs). DNAs were isolated from 35 pairs of skin tumors (25 BCCs and 10 SCCs) and matching normal skin from the same patients and analyzed for c‐Ha‐rasgene polymorphism by Southern blot hybridization. Sixteen BCC patients and 1 SCC patient were constitutionally heterozygous for the c‐Ha‐rasgene in their normal skin DNA. Of these 17 patients, five patients (four with BCC and one with SCC) (29%) showed loss of one of the c‐Ha‐rasalleles in their tumor DNA. One of the constitutionally heterozygous BCC patients exhibited deletion of the 6.6‐kb c‐Ha‐rasallele and an extra copy of the 7.8‐kb allele. In summary, loss of heterozygosity at the c‐Ha‐raslocus occurred frequently (29%) in the 17 human skin cancers studied. However, our finding that 90% of the patients with SCC, as opposed to 36% of the patients with BCC, had only one of the c‐Ha‐rasalleles in their normal skin tissue requires further study. Whether c‐Ha‐rashomozygosity has any bearing on genetic susceptibility to SCC remains to be established.