Defective Development of Sensory Neurons Innervating the Levator Ani Muscle in Fetal Rats with Anorectal Malformation
Defective Development of Sensory Neurons Innervating the Levator Ani Muscle in Fetal Rats with Anorectal Malformation
复制标题
肛门直肠畸形胎鼠支配肛提肌的感觉神经元发育缺陷
DOI：
10.1002/bdra.20576
复制
发表时间：
2009-07-01
影响因子：
--
通讯作者：
Yuan, Zhengwei
中科院分区：
文献类型：
--
作者：
Guan, Kaoping;Li, Hui;Yuan, Zhengwei
背景：肛门直肠畸形(ARM)患者术后肛门直肠功能不良与提肛肌的盆神经支配缺陷有关。我们以前曾在有手臂的大鼠身上发现支配肛提肌的神经细胞发育不足。在这项研究中，我们调查了支配提肛肌的感觉神经元的发育是否存在缺陷。方法：用乙硫脲(ETU)诱发胎鼠上肢损伤。逆行示踪荧光金(FG)注入肛提肌。检查了腰椎脊髓全长的连续横切面。对FG标记的感觉神经元的数量进行评分，并比较有臂胎儿和正常胎儿之间的差异。结果：FG标记的感觉神经元支配提肌和肌肉的数量。在正常对照组胎儿中，无畸形、低位闭锁、高位闭锁、高位闭锁合并神经管畸形的胎儿分别为(均值+/-)11,804+/-2362，10,429+/-1708,2886+/-705,1026+/-425,964+/-445。有或无神经管缺陷的胎儿肛门闭锁的FG标记感觉神经元明显少于对照组和ETU喂养的无畸形胎儿(p<；0.05)。结论：支配提肛肌的感觉神经元缺陷是胎儿发育过程中与上肢消化道异常并存的原发异常。盆腔肌的神经支配缺陷是ARM患者术后肛门直肠功能低下的原因之一。出生缺陷研究(A部分)85：583-587,2009。(C)2009年Wiley-Liss，Inc.
BACKGROUND: Defects of the pelvic nerve innervation of levator ani muscle are associated with poor postoperative anorectal function in patients with anorectal malformation (ARM). We have previously shown deficient development of rnotoneurons innervating the levator ani muscle in rats with ARM. In this study we investigate whether there is a deficiency in the development of sensory neurons that innervate the levator ani muscle in rats with ARM. METHODS: ARM Leas induced by ethylenethiourea (ETU) in fetal rats. Retrograde tracer fluorogold (FG) was injected into the levator ani muscle. Serial transverse sections encompassing the entire length of the lumbosacral spinal cord were examined. The number of FG-labeled sensory neurons was scored and compared between fetuses with ARM and normal fetuses. RESULTS: The number of FG-labeled sensory neurons innervating the levator and muscle. In normal control fetuses, ETU-fed fetuses with no malformation, low type of imperforate anus, high type of imperforate anus, and high type of imperforate anus combined with neural tube defects were determined to be (mean +/- SEM) 11,804 +/- 2362, 10,429 +/- 1708, 2886 +/- 705, 1026 +/- 425, and 964 +/- 445, respectively. FG-labeled sensory neurons in fetuses With imperforate anus with or Without neural tube defects were significantly fewer than in control and ETU-fed fetuses without malformation (p < 0.05). CONCLUSIONS: Defective sensory neurons innervating the levator ani muscle is a primary anomaly that coexists with the alimentary tract anomaly in ARM during fetal development. Nerve innervation deficiency of the pelvic muscles contributes to the poor postoperative anorectal functions in ARM patients. Birth Defects Research (Part A) 85:583-587, 2009. (C) 2009 Wiley-Liss, Inc.