Comparative proteomic analysis of indica and japonica rice varieties.
Comparative proteomic analysis of indica and japonica rice varieties.
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籼稻和粳稻品种的比较蛋白质组分析
DOI：
10.1590/s1415-47572014005000015
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发表时间：
2014-10
影响因子：
2.1
通讯作者：
Chen K
中科院分区：
文献类型：
--
作者：
Yang Y;Zhu K;Xia H;Chen L;Chen K
亚种是亚洲栽培稻的两个主要亚种。它们在形态和农艺性状、生理生化特性和基因组结构上都有明显的差异。然而，造成这些差异的蛋白质和基因仍然没有得到很好的描述。在这项研究中，蛋白质组学工具，包括双向电泳和质谱学，被用来在全球范围内鉴定两个测序水稻品种(93-11和日本晴)之间的差异蛋白质。通过质谱学和数据库搜索，总共鉴定出了在93-11和日本晴之间有显著差异的47种蛋白质。有趣的是，7个蛋白质仅在日本晴中表达，1个蛋白质在93-11中特异表达；这些差异在实时定量聚合酶链式反应和对其他水稻品种的蛋白质组学分析中得到了证实。这是首次成功地揭示了不同水稻品种间蛋白质组成的差异，并为进一步研究它们在水稻分化中的作用提供了候选蛋白质和基因。
Indica and japonica are two main subspecies of Asian cultivated rice (Oryza sativa L.) that differ clearly in morphological and agronomic traits, in physiological and biochemical characteristics and in their genomic structure. However, the proteins and genes responsible for these differences remain poorly characterized. In this study, proteomic tools, including two-dimensional electrophoresis and mass spectrometry, were used to globally identify proteins that differed between two sequenced rice varieties (93–11 and Nipponbare). In all, 47 proteins that differed significantly between 93–11 and Nipponbare were identified using mass spectrometry and database searches. Interestingly, seven proteins were expressed only in Nipponbare and one protein was expressed specifically in 93–11; these differences were confirmed by quantitative real-time PCR and proteomic analysis of other indica and japonica rice varieties. This is the first report to successfully demonstrate differences in the protein composition of indica and japonica rice varieties and to identify candidate proteins and genes for future investigation of their roles in the differentiation of indica and japonica rice.