Effect of dentin primers on the morphology, molecular composition and collagen conformation of acid-demineralized dentin in situ
Effect of dentin primers on the morphology, molecular composition and collagen conformation of acid-demineralized dentin in situ
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DOI：
10.1016/s0109-5641(99)00050-0
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发表时间：
1999-09-01
期刊：
影响因子：
5
通讯作者：
Palaghias, G
中科院分区：
文献类型：
--
作者：
Eliades, G;Vougiouklakis, G;Palaghias, G
目的：本研究的目的是评估两种商业引物和蒸馏水对酸脱矿dentinsitu.Methods的形态，分子组成和胶原蛋白构象的影响：牙本质标本蚀刻Scotchbond蚀刻剂的轻敲模式AFM成像和分析的MIR-FTIR光谱。然后用Scotchbond Multi-Purpose Plus Primer、Scotchbond 1 Adhesive或蒸馏水对其进行预处理，并再次成像和分析。在水和原始引物扣除后，研究了在最上面2 μ m的预处理牙本质上诱导的化学修饰。通过对酰胺I带成分进行去卷积，评价了I型胶原在该区域的构象变化。吸光度比值A（n）（1655/1627）用于半定量评估胶原变性的相对程度。预处理后没有发现初级粘合的证据，而大约50%的条件牙本质胶原蛋白出现变性。用蒸馏水处理没有改变变性胶原的状态，但是，应用的商业引物refolded的α-螺旋接近95%的程度上发现的本地参考dentin.Significance：牙本质胶原的动态响应脱矿和引发治疗揭示了一些引物在快速恢复酸变性胶原的构象状态的关键作用。在α-螺旋恢复中实施反应性粘合剂基团可以提供基于其分子间键合的程度改变脱矿物质胶原的机械性质的相关手段。(C)1999年牙科材料学院。由Elsevier Science Ltd.出版，版权所有。
Objectives: The purpose of this study was to assess the effect of two commercial primers and distilled water on the morphology, molecular composition and collagen conformation of acid-demineralized dentin in situ.Methods: Dentin specimens etched with Scotchbond Etchant were imaged by tapping mode AFM and analyzed by MIR-FTIR spectroscopy. They were then primed with Scotchbond Multi-Purpose Plus Primer, Scotchbond 1 Adhesive or distilled water and imaged and analyzed again. The chemical modifications induced on the uppermost 2 mu m of primed dentin were studied after water and original primer subtraction. The conformational changes of type I collagen at this region were evaluated by deconvoluting the amide I band components. The absorbance ratio A(n)(1655/1627) was used to semiquantitatively assess, on a relative basis, the extent of collagen denaturation.Results: All the priming treatments swelled the collapsed dentin collagen left after etching. No evidence of primary bonding was found after priming treatments, while approximately 50% of the conditioned dentin collagen appeared denatured. Treatment with distilled water did not change the status of denatured collagen, however, application of the commercial primers refolded the alpha-helix to approximate to 95% of the extent found on the native reference dentin.Significance: The dynamic response of dentin collagen to demineralization and priming treatments reveals the critical role of some primers in rapidly restoring the conformational status of acid-denatured collagen. Implementation of reactive adhesive groups in alpha-helix recovery may provide an associative means of modifying the mechanical properties of the demineralized collagen based on the extent of their intermolecular bonding. (C) 1999 Academy of Dental Materials. Published by Elsevier Science Ltd. All rights reserved.