Current Methods to Unravel the Functional Properties of Lysosomal Ion Channels and Transporters.
Current Methods to Unravel the Functional Properties of Lysosomal Ion Channels and Transporters.
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DOI：
10.3390/cells11060921
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发表时间：
2022-03-08
期刊：
影响因子：
6
通讯作者：
Carpaneto A
中科院分区：
文献类型：
--
作者：
Festa M;Minicozzi V;Boccaccio A;Lagostena L;Gradogna A;Qi T;Costa A;Larisch N;Hamamoto S;Pedrazzini E;Milenkovic S;Scholz-Starke J;Ceccarelli M;Vitale A;Dietrich P;Uozumi N;Gambale F;Carpaneto A
一组独特的通道和转运蛋白调节穿过溶酶体膜的离子通量。这些转运蛋白的功能障碍以及由此产生的离子失衡与多种人类疾病有关，例如溶酶体储存障碍、癌症以及代谢和神经退行性疾病。因此，这些蛋白质引起了人们对其作为可能的药物靶点的强烈兴趣。缺乏许多溶酶体通道和转运蛋白的详细功能表征，主要是由于将标准膜片钳技术应用于这些小的细胞内区室的技术困难。在这篇综述中，我们重点关注当前用于揭示溶酶体离子通道和转运蛋白功能特性的方法，强调它们的优点和缺点，并评估它们的适用领域。
A distinct set of channels and transporters regulates the ion fluxes across the lysosomal membrane. Malfunctioning of these transport proteins and the resulting ionic imbalance is involved in various human diseases, such as lysosomal storage disorders, cancer, as well as metabolic and neurodegenerative diseases. As a consequence, these proteins have stimulated strong interest for their suitability as possible drug targets. A detailed functional characterization of many lysosomal channels and transporters is lacking, mainly due to technical difficulties in applying the standard patch-clamp technique to these small intracellular compartments. In this review, we focus on current methods used to unravel the functional properties of lysosomal ion channels and transporters, stressing their advantages and disadvantages and evaluating their fields of applicability.
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DOI：
10.1074/jbc.m110.162073
发表时间：
2010-12-03
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Brailoiu E;Rahman T;Churamani D;Prole DL;Brailoiu GC;Hooper R;Taylor CW;Patel S
通讯作者：
Patel S
影响因子：
11.8
作者：
Cao, Qi;Zhong, Xi Zoe;Dong, Xian-Ping
通讯作者：
Dong, Xian-Ping
影响因子：
4.8
作者：
Carpaneto, A;Geiger, D;Hedrich, R
通讯作者：
Hedrich, R
影响因子：
5.1
作者：
Bregante, Monica;Yang, Yingzhen;Costa, Alex
通讯作者：
Costa, Alex
影响因子：
8
作者：
Boccaccio, Anna;Scholz-Starke, Joachim;Carpaneto, Armando
通讯作者：
Carpaneto, Armando