Airway epithelial damage induced by sulfur mustard in guinea pigs, effects of glucocorticoids
Airway epithelial damage induced by sulfur mustard in guinea pigs, effects of glucocorticoids
复制标题
DOI：
10.1177/096032719601501204
复制
发表时间：
1996-12-01
影响因子：
2.8
通讯作者：
Escudier, E
中科院分区：
文献类型：
--
作者：
Calvet, JH;Coste, A;Escudier, E
芥子气（SM）是一种潜在的化学战剂。为了研究SM对豚鼠气道上皮的损伤，我们研究了SM对豚鼠气管内注射0.3mg/kg SM后5 h、24 h、14 d和35 d的影响。在急性期，气道上皮主要表现为上皮内水疱、炎性细胞浸润和柱状细胞脱落，基底细胞暴露。中毒后14天，气管上皮出现紊乱，高度和细胞密度显着下降。SM中毒后35天，气管上皮细胞仍未完全恢复。第14天，在第7天至第14天接受倍他米松治疗的SM中毒豚鼠中，与未治疗的豚鼠相比，上皮高度、细胞密度和细胞增殖（通过免疫组织化学评价）显着增加。总之，在SM中毒豚鼠中观察到的病变似乎与临床人类观察结果一致，并且与SM诱导的气道上皮损伤的研究相关。该动物模型可用于说明主要来源于基底细胞的气管上皮再生，并显示糖皮质激素对化学侵袭后气道上皮恢复的影响。
Sulfur mustard (SM) represents a potential chemical warfare agent. In order to characterize SM-induced airway epithelial damage, we studied the effects of an intratracheal injection of 0.3 mg/kg of SM in guinea pigs, 5 h, 24 h, 14 days and 35 days after exposure, During the acute period, lesions prevailed in tracheal epithelium exhibiting intra-epithelial blisters, inflammatory cell infiltration and columnar cell shedding with exposure of basal cells. Fourteen days after intoxication, tracheal epithelium appeared disorganized and showed a significant decrease in height and cell density. Tracheal epithelium recovery was still not complete even 35 days after SM-intoxication. At day 14, in SM-intoxicated guinea pigs treated with betamethasone from day 7 to day 14, epithelium height, cell density and cell proliferation (evaluated by immunohistochemistry) were significantly increased compared to untreated guinea pigs. In conclusion, the lesions observed in SM-intoxicated guinea pigs seem to be in accordance with clinical human observations and are relevant to the study of airway epithelial damage induced by SM. This animal model could be used to illustrate tracheal epithelium regeneration mainly derived from basal cells and to show glucocorticoid effects on airway epithelial recovery after chemical aggression.