The frequency dependence of osmo-adaptation in Saccharomyces cerevisiae
The frequency dependence of osmo-adaptation in Saccharomyces cerevisiae
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DOI：
10.1126/science.1151582
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发表时间：
2008-01-25
期刊：
影响因子：
56.9
通讯作者：
van Oudenaarden, Alexander
中科院分区：
文献类型：
--
作者：
Mettetal, Jerome T.;Muzzey, Dale;van Oudenaarden, Alexander
信息通过信号级联的传递涵盖了广泛的时间尺度，包括快速的配体 - 受体相互作用以及下游基因表达慢得多的反应。为了确定哪种动态范围在反应中起主导作用，我们使用周期性刺激来测量酿酒酵母渗透适应途径中信号转导的频率依赖性。我们应用系统识别方法来推断一个简洁的预测模型。我们发现渗透适应反应的动力学由通过激酶Hog1的快速作用的负反馈所主导，该过程不需要蛋白质合成。在经历大的渗透冲击后，通过基因表达的另外一种慢得多的负反馈使细胞能够对未来的刺激更快地作出反应。
The propagation of information through signaling cascades spans a wide range of time scales, including the rapid ligand- receptor interaction and the much slower response of downstream gene expression. To determine which dynamic range dominates a response, we used periodic stimuli to measure the frequency dependence of signal transduction in the osmo- adaptation pathway of Saccharomyces cerevisiae. We applied system identification methods to infer a concise predictive model. We found that the dynamics of the osmo- adaptation response are dominated by a fast-acting negative feedback through the kinase Hog1 that does not require protein synthesis. After large osmotic shocks, an additional, much slower, negative feedback through gene expression allows cells to respond faster to future stimuli.