Subgenotype analysis of Cryptosporidium parvum isolates from humans and animals in Japan using the 60-kDa glycoprotein gene sequences
Subgenotype analysis of Cryptosporidium parvum isolates from humans and animals in Japan using the 60-kDa glycoprotein gene sequences
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DOI：
10.1007/s00436-006-0140-0
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发表时间：
2006-08-01
影响因子：
2
通讯作者：
Iseki, Motohiro
中科院分区：
文献类型：
--
作者：
Abe, Niichiro;Matsubayashi, Makoto;Iseki, Motohiro
微小隐孢子虫是一种常见的肠道寄生虫，与人类和动物的严重急性腹泻有关。该寄生虫由形态相同但遗传不同的多个基因型组成。在人类中，隐孢子虫病主要由两种C。parvum基因型，人类基因型（以前称为基因型1，最近被提出为新物种C.人）和牛基因型（以前称为基因型2）。然而，最近的分子生物学研究表明，C. parvum人或牛基因型。因此，在亚基因型水平上鉴定隐孢子虫分离株对控制隐孢子虫感染或了解隐孢子虫种群结构更有意义。parvum基因型。在本研究中，我们确定了C。使用C. parvum 60-kDa糖蛋白基因（GP 60），并显示了新的亚型在浣熊狗分离。本研究表明，C.小型牛基因型可能由人畜共患病和宿主特异性的多个亚基因型组成。
Cryptosporidium parvum is a well-known intestinal parasite which is associated with severe acute diarrhea in humans and animals. This parasite is composed of morphologically identical but genetically different multiple genotypes. In humans, cryptosporidiosis is mainly caused by two C. parvum genotypes, human genotype (previously known as genotype 1 and recently proposed as new species C. hominis) and cattle genotype (previously known as genotype 2). However, recent molecular studies indicate the genetic heterogeneity among the isolates of C. parvum human or cattle genotype. Therefore, identification of the isolates at the subgenotype level is more useful for control of the Cryptosporidium infection or for understanding of the population structure of C. parvum genotypes. In the present study, we identified the subgenotypes of the C. parvum human or cattle genotype isolates from humans and animals in Japan using DNA sequencing analysis of the C. parvum 60-kDa glycoprotein gene (GP60) and showed the new subgenotype in a raccoon dog isolate. This study suggested that C. parvum cattle genotype might be composed of zoonotic and host-specific multiple subgenotypes.