HUMAN MELANOCYTES CULTURED FROM NEVI AND MELANOMAS
HUMAN MELANOCYTES CULTURED FROM NEVI AND MELANOMAS
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DOI：
10.1111/1523-1747.ep12523594
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发表时间：
1986-07-01
影响因子：
6.5
通讯作者：
LERNER, AB
中科院分区：
文献类型：
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作者：
HALABAN, R;GHOSH, S;LERNER, AB
培养的人黑素细胞对生长因子的需求可能取决于恶性转化的阶段。几种因素协同促进培养的人新生黑素细胞的活力和增殖。它们是TPA（12-O-十四烷酰基-佛波醇-13-乙酸酯）、异丁基甲基黄嘌呤、霍乱毒素和来自几种细胞系和人胎盘的提取物的尚未鉴定的因子。新生儿的黑素细胞在6个月的时间内可以维持至少50个群体的增殖，而来自成人皮肤的黑素细胞仅增殖1个月，并且增殖率至少为来自新生儿包皮的黑素细胞的1%。相反，来自发育不良和先天性痣的黑素细胞在培养的最初4-6周内在有丝分裂原的存在下增殖良好，但随后变得静止。来自原发性黑色素瘤的黑素细胞是最难在培养中生长的。它们需要有丝分裂原，但它们的增殖速度很慢。大多数转移性黑素细胞株不需要有丝分裂原来增殖，被TPA强烈抑制，并且在较小程度上被WI-38细胞提取物抑制。我们的结论是，收购的独立性，从有丝分裂原在文化中是一个晚期事件的黑色素细胞转化为黑色素瘤。
The requirements for growth factors of human melanocytes in culture may be dependent on the stage of malignant transformation. Several factors synergistically promote the viability and proliferation of human neonatal melanocytes in culture. They are TPA (12-O-tetradecanoyl-phorbol-13-acetate), isobutylmethyl xanthine, cholera toxin, and as yet unidentified factors from extracts derived from several cell lines and human placenta. Neonatal melanocytes can maintain at least 50 population doublings during a period of 6 months, whereas melanocytes from adult skin proliferate only for 1 month and at least than 1% of the proliferative rate of melanocytes derived from newborn foreskins. In contrast, melanocytes from dysplastic and congenital nevi proliferate well in the presence of mitogens during the initial 4-6 weeks of culture, but then become quiescent. Melanocytes from primary melanomas are the most difficult to grow in culture. They need the mitogens, but their rate of proliferation is slow. Most of the metastatic melanocyte strains that do not need the mitogens in order to proliferate, are strongly inhibited by TPA, and to a lesser extent by WI-38 cell extract. We conclude that the acquisition of independence from mitogens in culture is a late event in the transformation of melanocytes to melanomas.