The effects of surface bioactivity and sustained-release of genistein from a mesoporous magnesium-calcium-silicate/PK composite stimulating cell responses in vitro, and promoting osteogenesis and enhancing osseointegration in vivo
The effects of surface bioactivity and sustained-release of genistein from a mesoporous magnesium-calcium-silicate/PK composite stimulating cell responses in vitro, and promoting osteogenesis and enhancing osseointegration in vivo
复制标题
介孔硅酸镁钙/PK复合材料的表面生物活性和金雀异黄素缓释作用在体外刺激细胞反应，在体内促进成骨和增强骨整合
DOI：
10.1039/c7bm01017f
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发表时间：
2018-04-01
影响因子：
6.6
通讯作者：
Su, Jiacan
中科院分区：
文献类型：
--
作者：
Cai, Liang;Zhang, Jue;Su, Jiacan
采用喷砂法对介孔镁钙硅酸盐(m-MCS)/聚醚醚酮(PK)复合材料(MPC)进行表面改性，并在改性后的MPC(MPCM)表面的m-MCS的纳米孔中负载染料木素(GS)。结果表明，与MPC相比，MPCM表面暴露的m-MCS增多，表面粗糙度和亲水性明显提高。此外，MPCM和GS负载的MPCM(MPCM-GE)的表面粗糙度和亲水性没有明显差异，并且两者在模拟体液溶液(SBF)中的磷灰石矿化能力都比MPC有所提高，表明了良好的表面生物活性。此外，与MPC相比，MPCM对MC3T3-E1细胞的黏附、增殖、分化和基因表达均有明显的刺激作用，GS从MPCM-GE表面的缓释进一步显著促进了细胞的增殖、分化和基因表达。根据Micro-CT、组织学和扫描电子显微镜的分析，结果表明，与MPC相比，MPCM明显促进了体内的成骨，促进了骨整合，并且从MPCM-GE表面释放的GS进一步促进了成骨，促进了骨整合。综上所述，体外细胞反应的显著促进，体内成骨能力的改善和骨整合的增强归因于表面生物活性和GS从MPCM-GE表面的缓释作用。因此，MPCM-GE将成为骨科和牙科应用的潜在候选材料。
The surface of a mesoporous magnesium-calcium-silicate (m-MCS)/polyetheretherketone (PK) composite (MPC) was modified by sand blasting, and genistein (GS) was loaded inside the nanopores of the m-MCS on the modified MPC (MPCm) surface. The results showed that compared with MPC, the surface roughness and hydrophilcity of MPCm obviously improved with more m-MCS exposed on its surface. Moreover, no obvious differences in surface roughness and hydrophilcity were found between MPCm and GS loaded MPCm (MPCm-Ge), and both of them possessed an improved apatite mineralization ability in simulated body fluid solution (SBF) compared with MPC, indicating excellent surface bioactivity. Moreover, the MPCm obviously stimulated the adhesion, proliferation, differentiation and gene expressions of MC3T3-E1 cells compared with MPC, and the sustained-release of GS from the MPCm-Ge surface further significantly promoted the cell proliferation, differentiation and gene expression. According to the Micro-CT, histological and SEM analysis, the results demonstrated that the MPCm obviously improved osteogenesis and enhanced osseointegration in vivo compared with MPC, and the release of GS from the MPCm-Ge surface further significantly improved osteogenesis and enhanced osseointegration. In summary, the significant promotion of cell responses in vitro, and the improvements of osteogenesis and the enhancement of osseointegration in vivo were attributed to the effects of surface bioactivity and GS sustained-release from the MPCm-Ge surface. Therefore, MPCm-Ge would be a potential candidate for orthopedic and dental applications.