ACCUMULATION OF PPGPP AND PPPGPP DURING NITROGEN DEPRIVATION OF THE CYANOPHYTE ANABAENA-CYLINDRICA
ACCUMULATION OF PPGPP AND PPPGPP DURING NITROGEN DEPRIVATION OF THE CYANOPHYTE ANABAENA-CYLINDRICA
复制标题
DOI：
10.1016/0014-5793(79)80489-5
复制
发表时间：
1979-01-01
期刊：
影响因子：
3.5
通讯作者：
SMITH, RJ
中科院分区：
文献类型：
--
作者：
AKINYANJU, J;SMITH, RJ
缺乏氨酰化 tRNA 会促进核糖体的异常反应，合成 S'-二磷酸、3'二磷酸鸟苷 (ppGpp) 和 5'。三磷酸3’。异养细菌中的二磷酸鸟苷 (pppGpp) [11。单细胞蓝藻 Anacystis nidulans 中也存在类似的机制 [2]，因此其积累模式与报道的异养细菌 [3-S] 相当。尽管对其他蓝藻积累 ppGpp 的能力知之甚少，但一项研究报告称，在 Aphanocapsa 6308、Anabaena cylindrica 和 Anabaena catenula 的入射光降级期间或在 Anabaena cylindrica 缺氮期间，没有 ppGpp 的积累 [2]。相反，该通讯报道了 A. cylindrica 积累核苷酸的能力，该核苷酸包含放射性标记的鸟苷和与从生长抑制的大肠杆菌中分离出的真实 ppGpp 在纸张、聚乙烯亚胺 (PEI) 纤维素薄层色谱 (TLC) 和高压液相色谱 (HPLC) 系统中共同迁移。核苷酸的积累是短暂的，并且在结合氮（特别是铵离子）存在的情况下很容易被抑制。
An anomalous reaction of the ribosome promoted by lack of aminoacylated tRNA synthesizes S’-diphosphate 3’diphosphate guanosine (ppGpp) and 5’. triphosphate 3’. diphosphate guanosine (pppGpp) in heterotrophic bacteria [11. A similar mechanism exists in the unicellular cyanophyte Anacystis nidulans [2], such that the mode of accumulation is comparable with that reported for heterotrophic bacteria [3-S]. Although little is known of the ability of other cyanophytes to accumulate ppGpp, one study reported no accumulation of ppGpp during incident light stepdown of Aphanocapsa 6308, Anabaena cylindrica and Anabaena catenula or during nitrogen deprivation of Anabaena cylindrica [2].In contrast, this communication reports on the ability of A. cylindrica to accumulate a nucleotide which incorporates radioactively labelled guanosine and co-migrates in paper, polyethyleneimine(PEI) cellulose thin-layer chromatography (TLC) and high pressure liquid chromatography (HPLC) systems with authentic ppGpp isolated from growth-inhibited E. coli. Accumulation of the nucleotide is short-lived and readily depressed in the presence of combined nitrogen, particularly ammonium ion.