Proteomics analysis reveals that CirA in Aeromonas hydrophila is involved in nutrient uptake
Proteomics analysis reveals that CirA in Aeromonas hydrophila is involved in nutrient uptake
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DOI：
10.1111/1462-2920.16336
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发表时间：
2023
期刊：
影响因子：
--
通讯作者：
Xiangmin Lin
中科院分区：
文献类型：
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作者：
Qingli Song;Lishan Zhang;Guibin Wang;Huanhuan Song;Shuangziying Zhang;Jindong Yao;Xiangmin Lin
The colicin I receptor (CirA) is a well-studied outer membrane protein that has been reported to play important roles in antibiotic resistance, virulence, and iron homeostasis, although its exact physiological roles require further investigation. In this study, differentially expressed proteins between the ΔahcirA and wild-type (WT) strains of Aeromonas hydrophila were compared using quantitative proteomics. Bioinformatics analysis revealed that the expression of peptide, histidine, and arginine ATP-binding cassette (ABC) transporter system-related proteins was significantly higher in the ΔahcirA strain. Subsequent growth assays revealed that ΔahcirA grew slower than the WT strain in nutrient-limited medium when supplemented with dipeptide, histidine, and arginine as the carbon source. Far-western blot analysis further confirmed that AhCirA can directly bind to histidine/arginine and dipeptide small-molecule substrates in addition to their periplasmic-binding proteins, AhDppA and AhHisJ, respectively. These results indicate that AhCirA may play an important role in the uptake of amino acids and peptides as a channel-forming porin while also directly interacting with ABC transporters to transport nutrient substances into the plasma membrane. Overall, this study demonstrates that AhCirA is a multifunctional protein in A. hydrophila and extends our understanding of known nutrient transport mechanisms among bacteria.
