CRISPR/Cas Based Biosensing Platforms for Molecular Diagnosis
CRISPR/Cas Based Biosensing Platforms for Molecular Diagnosis
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DOI：
10.19969/j.fxcsxb.21121601
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发表时间：
2022
期刊：
影响因子：
--
通讯作者：
Li Na
中科院分区：
文献类型：
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作者：
Li Tian;Li Na
Clustered regularly interspaced short palindromic repeats(CRISPR) and CRISPR-associated proteins (Cas)have become highly promising candidates in the construction of biosensing systems and diagnostic devices,in addition to their roles as revolutionary genome engineering tools.Forin vitro diagnostics,the class Ⅱ CRISPR/Cas systems possess unique advantages, such as high detection sensitivity and specificity, and the feasibility for developing point-of-care diagnostic technology. This minireview briefly introduces the working mechanism of CRISPR/Cas systems and mainly focus on the various diagnostic methods based on the CRISPR/Cas for detecting different forms of the genetic molecules in recent three years according to different readout methods.
