MONOCLONAL-ANTIBODIES TO CHOLERA-TOXIN WITH SPECIAL REFERENCE TO CROSS-REACTIONS WITH ESCHERICHIA-COLI HEAT-LABILE ENTERO-TOXIN
MONOCLONAL-ANTIBODIES TO CHOLERA-TOXIN WITH SPECIAL REFERENCE TO CROSS-REACTIONS WITH ESCHERICHIA-COLI HEAT-LABILE ENTERO-TOXIN
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DOI：
10.1128/iai.40.2.570-576.1983
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发表时间：
1983-01-01
影响因子：
3.1
通讯作者：
LYCKE, N
中科院分区：
文献类型：
--
作者：
LINDHOLM, L;HOLMGREN, J;LYCKE, N
制备了70种霍乱毒素单克隆抗体并进行了鉴定。IgG同型39例（IgG1, 29例IgG2, 2例IgG3）。61个克隆产生针对B亚基的抗体；9个克隆产生了针对霍乱毒素A亚基的特异性抗体。在抗b抗体和抗a抗体中，与大肠杆菌热不稳定肠毒素完全交叉反应的有14个克隆，与部分交叉反应的有12个克隆，与不交叉反应的有44个克隆。虽然25个测试的抗b单克隆抗体中有24个能中和霍乱毒素，但9个抗a克隆中没有一个具有可检测到的中和能力。在抗b抗体中，与大肠杆菌热不稳定肠毒素完全交叉反应的抗体均具有较强的霍乱毒素中和能力；交叉反应性较弱或没有交叉反应性的药物，其中和效力的变化更大。单克隆抗体的分离可区分不同细菌来源的肠毒素，这表明开发免疫诊断方法的可能性，从而可以在腹泻病患者的粪便中检测物种特异性肠毒素。
Seventy monoclonal antibodies to cholera toxin were prepared and characterized. All were of IgG isotypes (39 IgG1, 29 IgG2 and 2 IgG3). A total of 61 clones produced antibody directed against the B subunit; 9 clones produced antibodies with specificity for the cholera toxin A subunit. Among the anti-B and anti-A antibodies, there were representatives which showed full cross-reactivity with the heat-labile enterotoxin of E. coli (14 clones), others which gave partial cross-reactions (12) and still others (44) which did not cross-react. Although 24 of 25 tested anti-B monoclonal antibodies neutralized cholera toxin, 0 of 9 anti-A clones had any detectable neutralizing ability. Among the anti-B antibodies, those which cross-reacted completely with E. coli heat-labile enterotoxin all had strong cholera toxin-neutralizing capacity; those with lesser or no degree of cross-reactivity varied more in their neutralizing potency. The isolation of monoclonal antibodies that distinguish between enterotoxins of different bacterial origin suggests the possibility of developing immunodiagnostic methods allowing species-specific enterotoxin detection in stools of patients with diarrheal disease.