ISOLATION OF A CDNA FOR HSF2 - EVIDENCE FOR 2 HEAT-SHOCK FACTOR GENES IN HUMANS
ISOLATION OF A CDNA FOR HSF2 - EVIDENCE FOR 2 HEAT-SHOCK FACTOR GENES IN HUMANS
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DOI：
10.1073/pnas.88.16.6911
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发表时间：
1991-08-01
影响因子：
11.1
通讯作者：
KINGSTON, RE
中科院分区：
文献类型：
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作者：
SCHUETZ, TJ;GALLO, GJ;KINGSTON, RE
热休克反应是由一个进化上保守的蛋白质热休克因子（HSF）的转录调控。本文报道了从HeLa细胞中纯化HSF的方法及部分肽段序列。该肽序列用于分离具有预测的开放阅读框的人cDNA，该开放阅读框与酿酒酵母和果蝇HSF的DNA结合结构域具有同源性。该cDNA指导蛋白质的合成，该蛋白质以与HeLa HSF相同的特异性结合热休克元件，并刺激从热休克启动子的转录。表达的蛋白质与抗HSF抗体交叉反应。然而，令人惊讶的是，该cDNA并不编码从纯化的HeLa HSF获得的所有肽。这些肽由Rabindran等人[Rabindran，S. K.，Giorgi，G.，Clos，J. & Wu，C.等人（1991）Proc. Acad. Sci. USA 88，6906-6910.]因此，似乎有一个人类热休克因子基因家族，并且至少有两个独立但相关的HSF蛋白调节人类的应激反应。
The heat shock response is transcriptionally regulated by an evolutionarily conserved protein termed heat shock factor (HSF). We report the purification to homogeneity and the partial peptide sequence of HSF from HeLa cells. The peptide sequence was used to isolate a human cDNA with a predicted open reading frame that has homology to the DNA binding domains of both Saccharomyces cerevisiae and Drosophila HSFs. The cDNA directs the synthesis of a protein that binds to the heat shock element with specificity identical to HeLa HSF and stimulates transcription from a heat shock promoter. The expressed protein cross-reacts with anti-HSF antibodies. Surprisingly, however, this cDNA does not encode all of the peptides obtained from purified HeLa HSF. These peptides are encoded by a distinct human cDNA, HSF1, described by Rabindran et al. [Rabindran, S. K., Giorgi, G., Clos, J. & Wu, C. (1991) Proc. Natl. Acad. Sci. USA 88, 6906-6910.] It therefore appears that there is a human heat shock factor gene family and that at least two separate but related HSF proteins regulate the stress response in humans.