Dynamics of the primary cilium in shear flow.
Dynamics of the primary cilium in shear flow.
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DOI：
10.1016/j.bpj.2012.07.009
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发表时间：
2012-08
影响因子：
3.4
通讯作者：
Yuan-Nan Young;Matthew Downs;Christopher R. Jacobs
中科院分区：
文献类型：
--
作者：
Yuan-Nan Young;Matthew Downs;Christopher R. Jacobs
本文采用理论建模和实验相结合的方法，研究了初级纤毛在水流作用下的平衡形态和动力学。纤毛被模拟为弹性梁，可能由于流体动力载荷而经历大挠度。平衡结果表明，基底对纤毛轴丝的锚定作用表现为非线性旋转弹簧。通过将弹性梁与弹性壳耦合来阐明旋转弹簧的细节。我们进一步研究了由非线性旋转弹簧确定的纤毛基角在剪切流作用下纤毛的动力学，通过与实验结果的比较，得到了纤毛弯曲和松弛动力学的很好的一致性。这些结果潜在地阐明了通过睫状膜的机械敏感离子通道传输的物理基础。
In this work, the equilibrium shape and dynamics of a primary cilium under flow are investigated by using both theoretical modeling and experiment. The cilium is modeled as an elastic beam that may undergo large deflection due to the hydrodynamic load. Equilibrium results show that the anchoring effects of the basal body on the cilium axoneme behave as a nonlinear rotational spring. Details of the rotational spring are elucidated by coupling the elastic beam with an elastic shell. We further study the dynamics of cilium under shear flow with the cilium base angle determined from the nonlinear rotational spring, and obtain good agreement in cilium bending and relaxing dynamics when comparing between modeling and experimental results. These results potentially shed light on the physics underlying the mechanosensitive ion channel transport through the ciliary membrane.