Distribution and Speciation of Iron and Zinc in Grain of Two Wheat Genotypes
Distribution and Speciation of Iron and Zinc in Grain of Two Wheat Genotypes
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DOI：
10.1021/jf403331p
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发表时间：
2014-01-22
影响因子：
6.1
通讯作者：
Zhao, Fang-Jie
中科院分区：
文献类型：
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作者：
Eagling, Tristan;Neal, Andrew L.;Zhao, Fang-Jie
本研究旨在确定不同品种小麦籽粒中铁、锌的分布和形态差异。全麦粉中铁、锌含量较高的品种，白粉中铁、锌含量也较高。提取可溶性铁和锌，用体积排除-电感耦合等离子体质谱对其进行分析。铁的形态在不同的研磨组分之间不同，低分子量(LMW)络合物可能是铁-脱氧致突变酸/烟胺是白面粉中主要的可提取铁形态，约占可提取铁的85%。麸皮部分含有较少的低分子量铁，但更多的是以可溶性植酸铁的形式存在，并有一个未知的高分子量峰。在白粉组分中，可溶性锌主要以低分子量峰形式存在，可能是锌-烟胺络合物。在高铁品种的白粉中发现了可溶性植酸铁，而在低铁品种中没有。
This study aimed to determine differences among wheat cultivars in the distribution and speciation of Fe and Zn in grain milling fractions. Cultivars with higher Fe and Zn concentrations in the wholemeal flour were found to contain higher concentrations in the white flour. Soluble Fe and Zn were extracted and analyzed by size exclusion-inductively coupled plasma mass spectrometry. Fe speciation varied between milling fractions with a low molecular weight (LMW) complex likely to be Fe- deoxymugenic acid/nicotianamine being the predominant extractable Fe species in white flour, accounting for approximately 85% of the extractable Fe. Bran fractions had a lower amount of LMW-Fe form but more as soluble Fe phytate and an unidentified high molecular weight peak. In the white flour fraction soluble Zn was found to be present mainly as a LMW peak likely to be Zn-nicotianamine complex. Soluble Fe-phytate was found in the white flour fraction of a high-Fe cultivar but not in a low-Fe cultivar.