Ultrasound-Assisted Saponification Coupled with Gas Chromatography-Flame Ionization Detection for the Determination of Phytosterols from Passion Fruit Seed Oil
Ultrasound-Assisted Saponification Coupled with Gas Chromatography-Flame Ionization Detection for the Determination of Phytosterols from Passion Fruit Seed Oil
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DOI：
10.21577/0103-5053.20170170
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发表时间：
2018-03-01
影响因子：
1.4
通讯作者：
Visentainer, Jesuí V.
中科院分区：
文献类型：
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作者：
Rotta, Eliza M.;Silva, Michele C. da;Visentainer, Jesuí V.
建立了超声波辅助皂化（UAS）法测定西番莲籽油中植物甾醇（菜油甾醇、β-谷甾醇和豆甾醇）的方法，并对该方法进行了验证。在最佳条件下，所有分析物均呈良好的线性，相关系数均大于0.99。所提出的方法的回收率为103和107%之间，定量限为70和180 μ g kg（-1）和表示的相对标准偏差（RSD）的精密度值低于19%。该方法成功地应用于不同品种西番莲籽油样品的分析，包括黄色西番莲，甜石榴和甜西番莲。在所有分析的西番莲籽油中，发现β-谷甾醇的丰度最高，范围为0.90 +/- 0.02至1.60 +/- 0.01 mg g（-1）油，其次是豆甾醇（0.70 +/- 0.01至1.40 +/- 0.12 mg g（-1）油）和菜油甾醇（0.10 +/- 0.01至0.30 +/- 0.06 mg g（-1）油）。此外，所开发的方法被证明是一个节省时间和有效的分析程序，用于测定油样中的植物甾醇。
An ultrasound-assisted saponification (UAS) method for the determination of phytosterols (campesterol, beta-sitosterol and stigmasterol) in passion fruit seed oil, followed by gas chromatography with flame ionization detection, was developed and validated. Under the optimum conditions, good linearity with correlation coefficients higher than 0.99 was obtained for all analytes. The recoveries for the proposed approach were between 103 and 107%, the limits of quantification were between 70 and 180 mu g kg(-1) and the precision values expressed in terms of relative standard deviations (RSD) were lower than 19%. The developed method was successfully applied to the analysis of passion fruit seed oil samples from different species, including yellow passion fruit, sweet granadilla and sweet passion fruit. In all species of analyzed passion fruit seed oils, beta-sitosterol was found with the highest abundance, ranging from 0.90 +/- 0.02 to 1.60 +/- 0.01 mg g(-1) of oil, followed by stigmasterol (0.70 +/- 0.01 to 1.40 +/- 0.12 mg g(-1) of oil) and campesterol (0.10 +/- 0.01 to 0.30 +/- 0.06 mg g(-1) of oil). Furthermore, the developed method proved to be a time-saving and efficient analytical procedure for the determination of phytosterols in oil samples.