Triclosan (TCS) exposure impairs lipid metabolism in zebrafish embryos
Triclosan (TCS) exposure impairs lipid metabolism in zebrafish embryos
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DOI：
10.1016/j.aquatox.2016.01.001
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发表时间：
2016-04-01
影响因子：
4.5
通讯作者：
Tse, William K. F.
中科院分区：
文献类型：
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作者：
Ho, Jeff C. H.;Hsiao, C. D.;Tse, William K. F.
三氯生（TCS）是一种有效的抗菌成分，用于许多家用产品，如护肤霜和牙膏。它大量产生，人类直接接触到它，并每天处理它。TCS已被发现污染世界各地的水。本研究以斑马鱼为实验模型，旨在了解TCS暴露可能引起的发育和代谢异常。选择四个发育阶段（70-85%表观代谢，10-12 somite， prim-5和5dpf）进行原位杂交染色，以研究TCS对背腹侧图案，分割，大脑发育和器官形成的影响。结果表明，在发育毒理学方面，250 μ g/l TCS暴露5 d后未发现表型和分子变化。然而，这种剂量的TCS暴露导致卵黄囊内脂滴积累，这可能是由于β -氧化转录本mRNA表达水平失调所致。本研究表明，暴露于250 μ g/l的TCS不影响正常胚胎发生或器官发生；然而，有可能损害脂质代谢的担忧。(C) 2016 Elsevier B.V.版权所有
Triclosan (TCS) is an active antimicrobial ingredient used in many household products, such as skin creams and toothpaste. It is produced in high volumes, and humans are directly exposed to it and dispose it on a daily basis. TCS has been found to contaminate water worldwide. This study aimed to understand the potential developmental and metabolic abnormalities caused by TCS exposure by using zebrafish as the experimental model. Four developmental stages (70-85% epiboly, 10-12 somite, prim-5, and 5dpf) were selected to perform in situ hybridization staining to investigate the effects of TCS on dorsal ventral patterning, segmentation, brain development, and organ formation. Results showed, in terms of developmental toxicology, that neither phenotypic nor molecular changes were found after 5 days of 250 mu g/l TCS exposure. However, such dosage of TCS exposure resulted in lipid droplet accumulation in the yolk sac, which might due to the deregulated mRNA expression level of beta-oxidation transcripts. This study showed that 250 mu g/l, TCS exposure does not affect normal embryogenesis or organogenesis; however, there are concerns regarding possible impairment of lipid metabolism. (C) 2016 Elsevier B.V. All rights reserved.