Global profiling and characterization of Japanese flounder (Paralichthys olivaceus) kidney microRNAs regulated by Edwardsiella tarda infection in a time-dependent fashion
Global profiling and characterization of Japanese flounder (Paralichthys olivaceus) kidney microRNAs regulated by Edwardsiella tarda infection in a time-dependent fashion
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日本牙鲆（Paralichthys olivaceus）肾脏 microRNA 受迟缓爱德华氏菌感染以时间依赖性方式调节的整体分析和表征
DOI：
10.1016/j.fsi.2019.07.078
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发表时间：
2019-10-01
影响因子：
4.7
通讯作者：
Sun, Li
中科院分区：
文献类型：
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作者：
Li, Wen-rui;Hu, Yong-hua;Sun, Li
牙鲆（Paralichthysolivaceus）是我国和世界各国养殖的重要经济鱼类。它易受迟缓爱德华氏菌（Edwardsiella tarda）感染，这是一种宿主范围广泛的严重鱼类病原体。在这项研究中，我们采用高通量深度测序技术，在全球范围内鉴定了由E.在感染的不同阶段。差异表达的miRNAs（DEmiRNAs）和mRNA（DEmRNAs）在E.检查迟发性感染。共鉴定出96个DEmiRNAs，预测了2779个靶基因。87个miRNA-mRNA对，包括29个DEmiRNAs和86个DEmRNAs，其表达模式呈负相关。GO和KEGG富集分析显示DEmiRNAs的推定靶基因与多种生物学过程、细胞组分和分子功能相关。其中一种DEmiRNAs，pol-miR-182- 5 p，被证明以依赖于polmiR-182- 5 p的种子序列和PoS 1 PR 1的3 '-UTR之间的特异性相互作用的方式负调控鞘氨醇-1-磷酸受体1（PoS 1 PR 1）。pol-miR-182- 5 p在比目鱼细胞中的过表达促进细胞凋亡并抑制细胞活力。在比目鱼增强E.在鱼组织中的迟发性侵入和传播。这些结果为比目鱼miRNA介导的抗菌免疫提供了新的见解。
Japanese flounder (Paralichthys olivaceus) is an important economic fish species farmed in China and other countries. It is susceptible to infection by Edwardsiella tarda, a severe fish pathogen with a broad host range. In this study, we employed high-throughput deep sequencing technology to identify, in a global scale, flounder kidney microRNAs (miRNAs) induced by E. tarda at different stages of infection. Differentially expressed miRNAs (DEmiRNAs) and mRNAs (DEmRNAs) exhibiting significantly altered expression levels before and after E. tarda infection were examined. A total of 96 DEmiRNAs were identified, for which 2779 target genes were predicted. Eighty-seven miRNA-mRNA pairs, involving 29 DEmiRNAs and 86 DEmRNAs, showed negative correlations in their expression patterns. GO and KEGG enrichment analysis revealed that the putative target genes of the DEmiRNAs were associated with diverse biological processes, cellular components, and molecular functions. One of the DEmiRNAs, pol-miR-182-5p, was demonstrated to regulate sphingosine-1-phosphate receptor 1 (PoS1PR1) negatively in a manner that depended on the specific interaction between the seed sequence of polmiR-182-5p and the 3'-UTR of PoS1PR1. Overexpression of pol-miR-182-5p in flounder cells promoted apoptosis and inhibited cellular viability. Knockdown of PoS1PR1 in flounder enhanced E. tarda invasion and dissemination in fish tissues. These results provide new insights into miRNA-mediated anti-bacterial immunity in flounder.