Immobilization of Biomolecules on Poly(vinyldimethylazlactone)-Containing Surface Scaffolds
Immobilization of Biomolecules on Poly(vinyldimethylazlactone)-Containing Surface Scaffolds
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DOI：
10.1021/la802925g
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发表时间：
2009-01-06
期刊：
影响因子：
3.9
通讯作者：
Kilbey, S. Michael, II
中科院分区：
文献类型：
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作者：
Barringer, Joshua E.;Messman, Jamie M.;Kilbey, S. Michael, II
我们描述了在硅片上逐步形成反应性多层聚合物支架的成功开发过程，该支架包含基于2-乙烯基-4,4-二甲基内酯（VDMA）的聚合物，并对这些材料进行了表征。还讨论了一种将生物分子非位点特异性附着到改性硅片上的方法的发展，包括通过按需滴注（DOD）喷墨打印修饰的支架。基于vdma的聚合物具有水解稳定性，并且其悬垂的内酯环能够通过亲核加成与伯胺形成稳定的共价键而不产生副产物。该反应无需催化剂，在室温下进行，产生稳定的酰胺链，这增加了使用vdma基聚合物时所期望的构建便利性。由于图案设计的灵活性和易用性，DOD喷墨打印作为一种有趣的方法被探索用于创建具有一种或多种生物分子图案的表面。
We describe the successful development of a procedure for the step-by-step formation of a reactive, multilayer polymer scaffold incorporating polymers based on 2-vinyl-4,4-dimethylazlactone (VDMA) on a silicon wafer and the characterization of these materials. Also discussed is the development of a procedure for the nonsite specific attachment of a biomolecule to a modified silicon wafer, including scaffolds modified via drop-on-demand (DOD) inkjet printing. VDMA-based polymers were used because of their hydrolytic stability and ability of the pendant azlactone rings to form stable covalent bonds with primary amines without byproducts via nucleophilic addition. This reaction proceeds without a catalyst and at room temperature, yielding a stable amide linkage, which adds to the ease of construction expected when using VDMA-based polymers. DOD inkjet printing was explored as an interesting method for creating surfaces with one or more patterns of biomolecules because of the flexibility and ease of pattern design.