Establishment of a ciliated epithelial cell line from human Fallopian tube
Establishment of a ciliated epithelial cell line from human Fallopian tube
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DOI：
10.1093/humrep/15.7.1597
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发表时间：
2000-07-01
影响因子：
6.1
通讯作者：
Mizutani, S
中科院分区：
文献类型：
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作者：
Ando, H;Kobayashi, M;Mizutani, S
用猴病毒40（SV 40）大T抗原质粒转染原代培养的人输卵管上皮细胞，建立了无危象的永生化纤毛细胞系NT/T-S。透射电镜观察发现NT/T-S细胞具有纤毛、微绒毛、连接复合体、粗面内质网、游离核糖体和微管。本文对体外受精和胚胎移植中4 ~ 8-细胞期剩余胚胎进行了NT/T-S细胞共培养研究，初步评价了NT/T-S细胞的冷冻效果。133枚胚胎的碎片率均大于或等于10%（以表面积计），不适合冷冻保存。在含10-30%碎片的胚胎中，有57%（16/28）的胚胎经共培养后达到囊胚期。
Human tubal epithelial cells in primary culture were transfected with simian virus 40 (SV40) large T antigen plasmid, and an immortalized ciliated cell line, named as NT/T-S, was established without crisis. Transmission electron microscopy proved that NT/T-S cells had cilia, microvilli, junctional complexes, rough endoplasmic reticula, free ribosomes and microtubules. NT/T-S cells were evaluated preliminarily on the basis of co-culture study using surplus embryos at the 4- to 8-cell stage in our IVF and embryo transfer programme, All of the 133 embryos had greater than or equal to 10% fragments (based on the surface area) and were unworthy of cryopreservation. Up to 57% (16/28) of the embryos with 10-30% fragments reached the blastocyst stage by co-culture, In contrast, blastocyst formation was observed in