ANOMALOUS SIDE-CHAIN AMIDATION IN PLASMA-MEMBRANE PROTEINS OF SIMIAN VIRUS 40-TRANSFORMED LYMPHOCYTES INDICATED BY ISOELECTRIC FOCUSING AND LASER RAMAN-SPECTROSCOPY
ANOMALOUS SIDE-CHAIN AMIDATION IN PLASMA-MEMBRANE PROTEINS OF SIMIAN VIRUS 40-TRANSFORMED LYMPHOCYTES INDICATED BY ISOELECTRIC FOCUSING AND LASER RAMAN-SPECTROSCOPY
复制标题
DOI：
10.1016/0006-291x(75)90782-2
复制
发表时间：
1975-01-01
影响因子：
3.1
通讯作者：
WALLACH, DFH
中科院分区：
文献类型：
--
作者：
SCHMIDTULLRICH, R;VERMA, SP;WALLACH, DFH
比较了正常仓鼠淋巴细胞和SV 40 - 1转化淋巴细胞的质膜。在1%Triton X-100 8 M尿素中的等电聚焦显示转化细胞膜中非糖基化蛋白质的等电点高于正常。这表明膜谷氨酸盐和/或谷氨酸盐的酰胺化程度更高。聚焦模式还揭示了大多数糖基化蛋白质的等电点向较低pH的转变，表明增加的唾液酸化。两种膜类别的酰胺I-酰胺II激光拉曼光谱与肿瘤淋巴细胞膜中更大的侧链酰胺化一致。
Plasma membranes isolated from normal hamster lymphocytes and lymphoid cells transformed by SV40 1 have been compared. Isoelectric focussing in 1% Triton X-100 8M urea reveals higher isoelectric points than normal for the non-glycosylated proteins in the membranes of transformed cells. This suggests greater amidation of membrane aspartates and/or glutamates. The focussing patterns also reveal a shift to lower pH for the isoelectric points of most glycosylated proteins suggesting increased silalylation. The Amide I–Amide II laser Raman spectra of the two membrane categories are consistent with greater side chain amidation in the membranes of neoplastic lymphocytes.