Stable colloidal dispersions of C60 fullerenes in water:: Evidence for genotoxicity
Stable colloidal dispersions of C60 fullerenes in water:: Evidence for genotoxicity
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DOI：
10.1021/es0609708
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发表时间：
2006-12-01
影响因子：
11.4
通讯作者：
Tarabara, Volodymyr V.
中科院分区：
文献类型：
--
作者：
Dhawan, Alok;Taurozzi, Julian S.;Tarabara, Volodymyr V.
通过两种方法制备不含有毒有机溶剂的胶体C-60富勒烯的稳定水悬浮液：乙醇-水溶剂交换（EthOH/nC（60）悬浮液）和在水中延长混合（aqu/nC（60）悬浮液）。扩展混合方法导致形成比通过乙醇-水溶剂交换制备的nC（60）（（d）在棒（p）上约为122 nm，（xi）在棒上约为-31.6 mV）更大（（d）在棒（p）上约为178 nm）且带更少负电荷（（xi）在棒上约为-13.5 mV）的nC（60）胶体。使用单细胞凝胶电泳试验（彗星试验）评价这些混悬液对人淋巴细胞的遗传毒性。该试验证明了两种类型悬浮液的遗传毒性，遗传毒性反应与nC（60）浓度之间存在强相关性，并且在水/nC（60）低至2.2 μ g/L和EtOH/nC（60）低至4.2 μ g/L的浓度下观察到遗传毒性。这两种浓度的Olive尾矩（OTM）分别为1.54 +/- 0.24和1.34 +/- 0.07，与阴性对照OTM 0.98 +/- 0.17相比具有统计学差异，p值至少为0.05。对于相同的nC（60）浓度，Aqu/ nC（60）混悬液引起的遗传毒性反应高于EthOH/nC（60）混悬液。这些结果代表了通过简单混合在水中产生的胶体富勒烯的第一个遗传毒性数据。
Stable aqueous suspensions of colloidal C-60 fullerenes free of toxic organic solvents were prepared by two methods: ethanol to water solvent exchange (EthOH/nC(60) suspensions) and extended mixing in water (aqu/nC(60) suspensions). The extended mixing method resulted in the formation of larger ((d) over bar (p) approximate to 178 nm) and less negatively charged ((xi) over bar approximate to -13.5 mV) nC(60) colloids than nC(60) prepared by ethanol to water solvent exchange ((d) over bar (p) approximate to 122 nm, (xi) over bar approximate to - 31.6 mV). Genotoxicity of these suspensions was evaluated with respect to human lymphocytes using single-cell gel electrophoresis assay (Comet assay). The assay demonstrated genotoxicity for both types of suspensions with a strong correlation between the genotoxic response and nC(60) concentration, and with genotoxicity observed at concentrations as low as 2.2 mu g/L for aqu/nC(60) and 4.2 mu g/L for EtOH/nC(60). The Olive tail moments (OTM) for these two concentrations were 1.54 +/- 0.24 and 1.34 +/- 0.07, respectively, which in comparison to the negative control OTM of 0.98 +/- 0.17 is statistically different with a p value of at least 0.05. Aqu/ nC(60) suspensions elicited higher genotoxic response than EthOH/nC(60) for the same nC(60) concentration. The results represent the first genotoxicity data for colloidal fullerenes produced by simple mixing in water.