Light-based vat-polymerization bioprinting
Light-based vat-polymerization bioprinting
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DOI：
10.1038/s43586-023-00231-0
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发表时间：
2023-06-22
期刊：
影响因子：
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通讯作者：
Zhang, Yu Shrike
中科院分区：
文献类型：
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作者：
Levato, Riccardo;Dudaryeva, Oksana;Zhang, Yu Shrike
基于光的瓮聚合生物打印使得能够使用填充有可光活化的生物树脂（bioresins）的瓮（vats）以逐点、逐层或体积方式对载有细胞的3D结构进行计算机辅助图案化。这些技术的集合-按其操作模式分为立体光刻，数字光处理和体积增材制造-在过去几十年中得到了广泛的发展，导致了生物医学的广泛应用。在本书中，我们从硬件，软件和生物树脂选择的角度阐述了基于光的还原聚合3D生物打印的方法。我们随后讨论了这些技术的方法变化，包括其最新进展，并详细说明了用于确保生物打印程序和产品质量的关键评估。最后，我们提供了基于光的还原聚合方法的未来方向的见解。
Light-based vat-polymerization bioprinting enables computer-aided patterning of 3D cell-laden structures in a point-by-point, layer-by-layer or volumetric manner, using vat (vats) filled with photoactivatable bioresin (bioresins). This collection of technologies - divided by their modes of operation into stereolithography, digital light processing and volumetric additive manufacturing - has been extensively developed over the past few decades, leading to broad applications in biomedicine. In this Primer, we illustrate the methodology of light-based vat-polymerization 3D bioprinting from the perspectives of hardware, software and bioresin selections. We follow with discussions on methodological variations of these technologies, including their latest advancements, as well as elaborating on key assessments utilized towards ensuring qualities of the bioprinting procedures and products. We conclude by providing insights into future directions of light-based vat-polymerization methods.