Laminin receptors in the retina: sequence analysis of the chick integrin alpha 6 subunit. Evidence for transcriptional and posttranslational regulation.
Laminin receptors in the retina: sequence analysis of the chick integrin alpha 6 subunit. Evidence for transcriptional and posttranslational regulation.
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DOI：
10.1083/jcb.113.2.405
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发表时间：
1991-04
期刊：
影响因子：
--
通讯作者：
Reichardt LF
中科院分区：
文献类型：
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作者：
de Curtis I;Quaranta V;Tamura RN;Reichardt LF
整合素6 β 1是许多细胞类型使用的重要层粘连蛋白受体。在目前的工作中，我们分离克隆并确定鸡整合素α 6亚基的一级序列。我们发现α 6 β 1是发育中的鸡视网膜细胞表达的重要整合素。在胚胎第6天和第12天之间，视网膜神经节细胞和其他视网膜神经元都失去了特定的整合素功能，包括在层粘连蛋白上附着和延伸神经突的能力。在视网膜神经节细胞中，我们发现这与α 6 mRNA和蛋白的急剧减少有关，这表明基因表达的变化解释了这些神经元与层粘连蛋白相互作用的发育调节。在其他视网膜神经元中，α 6 mRNA和蛋白的表达仍然很高，但功能丧失，这表明α 6 β 1异源二聚体在这些细胞中的功能受到翻译后机制的调节。
The integrin alpha 6 beta 1 is a prominent laminin receptor used by many cell types. In the present work, we isolate clones and determine the primary sequence of the chick integrin alpha 6 subunit. We show that alpha 6 beta 1 is a prominent integrin expressed by cells in the developing chick retina. Between embryonic days 6 and 12, both retinal ganglion cells and other retinal neurons lose selected integrin functions, including the ability to attach and extend neurites on laminin. In retinal ganglion cells, we show that this is correlated with a dramatic decrease in alpha 6 mRNA and protein, suggesting that changes in gene expression account for the developmental regulation of the interactions of these neurons with laminin. In other retinal neurons the expression of alpha 6 mRNA and protein remains high while function is lost, suggesting that the function of the alpha 6 beta 1 heterodimer in these cells is regulated by posttranslational mechanisms.