DEFINED AGAR MEDIUM FOR GENETIC TRANSFORMATION OF NEISSERIA MENINGITIDIS
DEFINED AGAR MEDIUM FOR GENETIC TRANSFORMATION OF NEISSERIA MENINGITIDIS
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DOI：
10.1128/jb.83.3.470-474.1962
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发表时间：
1962-01-01
影响因子：
3.2
通讯作者：
SCHLOER, GM
中科院分区：
文献类型：
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作者：
CATLIN, BW;SCHLOER, GM
开发了一种琼脂培养基，用于脑膜炎奈瑟菌菌株15的定量遗传研究。它含有八种无机盐，柠檬酸钠，乳酸钠，精氨酸，半胱氨酸，甘氨酸，谷氨酸钠和纯化琼脂。在50次连续传代培养中，在没有添加二氧化碳的情况下获得了丰富的表面生长。亲本型菌落在确定培养基上的数量与在复杂培养基上的数量有密切的对应关系。细胞在确定的琼脂培养基上连续传代三或四次，并直接置于确定的液体培养基中转化脱氧核糖核酸的溶液中，无需额外补充即可进行转化。在处理过的群体中，0.1 - 0.3%的细胞转化为链霉素抗性。
An agar medium was developed for use in quantitative genetic studies of Neisseria meningitidis strain 15. It contains eight inorganic salts, sodium citrate, sodium lactate, arginine, cysteine, glycine sodium glutamate, and purified agar. Abundant surface growth in the ab-sence of supplemental carbon dioxide was obtained during 50 serial subcultures. A close correspondence was found between numbers of parental type colonies developing on the defined medium and on a complex medium. Cells subcultured serially three or four times on defined agar medium and placed directly into a solution of transforming deoxyribonucleic acid in defined liquid medium were susceptible to transformation without additional supplements. Of the treated population, 0.1 to 0.3% of the cells were transformed to streptomycin resistance.