Specific high-affinity binding protein for human corticotropin-releasing hormone in normal human plasma.
Specific high-affinity binding protein for human corticotropin-releasing hormone in normal human plasma.
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正常人血浆中人促肾上腺皮质激素释放激素的特异性高亲和力结合蛋白。
DOI：
10.1016/0006-291x(87)91369-6
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发表时间：
1987
影响因子：
3.1
通讯作者：
Mount,CD
中科院分区：
文献类型：
--
作者：
Orth,DN;Mount,CD
正常人血浆中Mr 25 -40千道尔顿的蛋白质结合人促肾上腺皮质激素释放激素（hCRH），但不结合绵羊CRH。结合需要N端和C端hCRH序列，Kd为2 × 10− 10 M，结合位点浓度为1.4 pmol/ml血浆。其结合不受加热至56°C持续1小时的影响，但通过暴露于6 M盐酸胍、10 mM二硫苏糖醇而消除。结合在37°C下迅速进行，在4分钟内完成75%。大鼠和绵羊血浆似乎都不含CRH结合蛋白。CRH结合蛋白可以解释hCRH与绵羊CRH相比在男性中的短暂生物学作用，以及为什么在妊娠晚期女性中高浓度的血浆免疫反应性hCRH不会引起ACTH分泌增加。
A protein of Mr25–40 kilodaltons in normal human plasma binds human corticotropin-releasing hormone (hCRH), but not ovine CRH. Binding requires both N- and C-terminal hCRH sequences and has a Kdof 2 × 10−10M and a binding site concentration of 1.4 pmoles per ml of plasma. Its binding is not affected by heating to 56°C for 1 h, but is abolished by exposure to 6 M guanidine-HCl, 10 mM dithiothreitol. Binding proceeds rapidly at 37°C and is 75% complete within 4 min. Neither rat nor sheep plasma appears to contain a CRH-binding protein. CRH-binding protein may explain the brief biological action of hCRH as compared to ovine CRH in man and why high concentrations of plasma immunoreactive hCRH in women during third-trimester pregnancy do not cause increased ACTH secretion.