Signature amino acid changes in latent membrane protein 1 distinguish Epstein-Barr virus strains
Signature amino acid changes in latent membrane protein 1 distinguish Epstein-Barr virus strains
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DOI：
10.1006/viro.1999.9855
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发表时间：
1999-08-15
期刊：
影响因子：
3.7
通讯作者：
Raab-Traub, N
中科院分区：
文献类型：
--
作者：
Edwards, RH;Seillier-Moiseiwitsch, F;Raab-Traub, N
EB病毒（EBV）潜伏膜蛋白1基因的序列变异已在许多EBV相关肿瘤中描述，其中一些变异，最值得注意的是细胞质羧基末端结构域中的30个碱基对缺失，表明与致瘤性增加相关。本研究对92例鼻咽癌、口腔毛状白斑、移植后淋巴瘤、移植后非病理性单核细胞增多症、伯基特淋巴瘤、腮腺肿瘤和不同地区正常人的组织标本或细胞系进行了EB病毒DNA序列测定。氨基和羧基末端序列，并在某些情况下，全长序列的潜伏膜蛋白1进行了测定。特征性序列模式区分菌株，羧基末端序列是区分菌株的最有用的信息。确定菌株之间的系统发育关系，以及区分它们的标志性氨基酸变化。菌株与疾病或菌株与地理位置之间的相关性未被检测到。序列变异和特征序列鉴定了至少七种不同的菌株，以及显然由重组产生的杂交菌株。(C)北京：科学出版社.
Sequence variations in the Epstein-Barr virus (EBV) latent membrane protein 1 gene have been described in numerous EBV-associated tumors with some of these variations, most notably a 30-base pair deletion in the cytoplasmic carboxyl-terminal domain, suggested as associated with an increase in tumorigenicity. In this study, EBV DNA sequence was determined from 92 tissue specimens or cell lines, including nasopharyngeal carcinoma, oral hairy leukoplakia, posttransplant lymphoma, post-transplant without pathology mononucleosis, Burkitt's lymphoma, parotid tumor, and normal from distinct geographical regions. The amino- and carboxyl-terminal sequences and, in some cases, the full-length sequences of latent membrane protein 1 were determined. Characteristic sequence patterns distinguished strains, with the carboxyl-terminal sequence being the most informative in distinguishing among the strains. Phylogenetic relationships between strains were determined, as were signature amino acid changes that discriminate between them. A correlation between strain and disease or strain and geographic location was not detected. The sequence variation and signature sequences identified at least seven distinct strains, as well as hybrid strains that apparently result from recombination. (C) 1999 Academic Press.