Growth of rumen papillae in weaned calves is associated with lower expression of insulin-like growth factor-binding proteins 2, 3, and 6
Growth of rumen papillae in weaned calves is associated with lower expression of insulin-like growth factor-binding proteins 2, 3, and 6
复制标题
DOI：
10.1111/asj.13270
复制
发表时间：
2019-07-10
影响因子：
2
通讯作者：
Roh, Sanggun
中科院分区：
文献类型：
--
作者：
Nishihara, Koki;Suzuki, Yutaka;Roh, Sanggun
本研究旨在探讨犊牛瘤胃乳头生长与瘤胃乳头中胰岛素样生长因子结合蛋白（igfbp） mRNA表达的关系。将9头荷斯坦犊牛分为哺乳组（2周，n = 3）、泌乳组（8周，n = 3）和断奶组（8周，n = 3），采用实时荧光定量PCR法检测瘤胃乳头长度、瘤胃乳头igfbp mRNA表达量，免疫组化（IHC）法检测胰岛素样生长因子I和II （IGF-I和II）含量。断奶犊牛瘤胃乳头长度比哺乳犊牛和泌乳犊牛长（p < 0.01），而IGFBP2、IGFBP3和IGFBP6基因表达量低于泌乳犊牛（p < 0.05）。因此，瘤胃乳头长度与IGFBP2、3和6的表达呈负相关。IHC分析显示犊牛瘤胃上皮中存在igf - 1和igf - 2。上述结果提示，断奶后瘤胃乳头的生长与低水平IGFBP2、IGFBP3和IGFBP6诱导的igf有关。
This study aimed to characterize the relationship between the growth of rumen papillae in calves and the mRNA expression of insulin-like growth factor-binding proteins (IGFBPs) in the rumen papillae. The length of rumen papillae, the mRNA expression of IGFBPs in rumen papillae by quantitative real-time PCR, and the presence of insulin-like growth factors I and II (IGF-I and II) by immunohistochemistry (IHC) were analyzed in nine Holstein calves divided into three groups: suckling (2 weeks, n = 3), milk-continued (8 weeks, n = 3), and weaned (8 weeks, n = 3). The length of rumen papillae was greater (p < 0.01) in weaned calves than in suckling and milk-continued calves, whereas the expressions of IGFBP2, IGFBP3, and IGFBP6 genes were lower (p < 0.05) in the rumen papillae of weaned calves than in milk-continued calves. Thus, rumen papillae length and IGFBP2, 3, and 6 expressions were negatively correlated. The IHC analysis showed that IGF-I and IGF-II were present in the rumen epithelium of calves. These results suggested that the growth of rumen papillae after weaning is associated with the induction of IGFs by the low levels of IGFBP2, IGFBP3, and IGFBP6.