Identification of Resistance Genes Using Diagnostic R-Gene Enrichment Sequencing (dRenSeq).

Identification of Resistance Genes Using Diagnostic R-Gene Enrichment Sequencing (dRenSeq).
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使用诊断 R 基因富集测序 (dRenSeq) 鉴定耐药基因。

DOI:
10.1007/978-1-0716-1609-3_10
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发表时间:
2021
期刊:
Methods in molecular biology (Clifton, N.J.)
影响因子:
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通讯作者:
Armstrong M
Armstrong M
中科院分区:
--
文献类型:
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作者:
Armstrong M

文献摘要

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诊断抗性基因富集测序(dRenSeq)是一种在复杂遗传背景下跟踪和验证多个功能性NLR基因完整性的方法。传统上,这些任务是通过费力和耗时的基于pcr的方法来解决的,或者在同源无毒基因可用的情况下,通过效应识别研究来解决。这两种方法在成本效益、效率和准确性方面都被dRenSeq所取代。
DiagnosticResistance geneenrichmentSequencing (dRenSeq) is a method for tracking and verifying the integrity of multiple functional NLR genes in complex genetic backgrounds. Traditionally, these tasks have been addressed by laborious and time-consuming PCR-based approaches or, where cognate avirulence genes are available, effector recognition studies. Both these approaches are superseded by dRenSeq in terms of cost-effectiveness, efficiency, and accuracy.