Use of flowering gene FLOWERING LOCUS T (FT) homologs in the phylogenetic analysis of bambusoid and early diverging grasses
Use of flowering gene FLOWERING LOCUS T (FT) homologs in the phylogenetic analysis of bambusoid and early diverging grasses
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DOI：
10.1007/s10265-008-0181-9
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发表时间：
2008-09-01
影响因子：
2.8
通讯作者：
Kobayashi, Mikio
中科院分区：
文献类型：
--
作者：
Hisamoto, Yoko;Kashiwagi, Harutsugu;Kobayashi, Mikio
从Phyllostachys meyeri 中克隆了一个核基因FLOWERING LOCUS T (FT) 同源物，名为PmFT。通过Southern印迹分析估计其假定的拷贝数为4，并且两个拷贝被完全测序。竹类和早期分化禾本科植物的 27 个 FT 同源序列包含 172 bp 外显子和 357 至 785 bp 内含子，表现出 0-58.9% 的成对分歧，具有 6 个模式水平。对植根于 Pharus virescens 的 FT 同系物进行简约分析，产生了六棵同样简约的树。在严格共识树中，解决了五个分支；它们受到内含子区域而不是外显子区域分歧的影响。基础进化枝是Puelioideae，其次是Olyreae 进化枝，包括Oryza sativa。 Streptogyneae 进化枝将 Olyreae 进化枝与竹科的末端姐妹进化枝（即泛热带竹子和东亚温带竹子）结合起来。全局拓扑表明 FT 同系物对于解决部落级别具有重要意义。然而，由于链霉菌分支的居间定位，FT同源物的系统发育在竹亚科中并不能解决单系问题。我们讨论了 FT 同源物在控制竹子系统发育中链霉菌的花序结构和位置中的作用。
A nuclear gene, FLOWERING LOCUS T (FT) homolog, was cloned from Phyllostachys meyeri as PmFT. Its putative copy number was estimated as four by Southern blot analysis, and the two copies were completely sequenced. Twenty-seven FT homolog sequences of bambusoid and early diverging grasses comprised 172-bp exons, and 357- to 785-bp introns exhibited 0-58.9% pairwise divergence with six modal levels. Parsimony analyses of the FT homologs rooted at Pharus virescens produced six equally parsimonious trees. In the strict consensus tree, five clades were resolved; they were affected by divergence of the intron region rather than exon region. The basal clade was Puelioideae, followed by Olyreae clade including Oryza sativa. Streptogyneae clade combined the Olyreae clade with terminal sister clades of the Bambuseae, i.e., pantropical bamboos and East Asiatic temperate bamboos. The global topology suggested that FT homologs are significant for resolving the tribe level. However, the phylogeny of FT homologs does not resolve monophyly in Bambusoideae because of intercalary positioning by Streptogyneae clade. We discussed the role of FT homologs in controlling the inflorescence architecture and position of Streptogyneae in the bamboo phylogeny.